γH2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity – preliminary methodological study and discussion

In order to improve patients’ post-treatment quality of life, a shift from surgery to non-surgical (chemo)radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still mi...

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Published in:The European physical journal. D, Atomic, molecular, and optical physics Atomic, molecular, and optical physics, 2017-09, Vol.71 (9), p.1-7, Article 241
Main Authors: Falk, Martin, Horakova, Zuzana, Svobodova, Marketa, Masarik, Michal, Kopecna, Olga, Gumulec, Jaromir, Raudenska, Martina, Depes, Daniel, Bacikova, Alena, Falkova, Iva, Binkova, Hana
Format: Article
Language:English
Subjects:
Applications of Nonlinear Dynamics and Chaos Theory
Atomic
Deoxyribonucleic acid
DNA
Fibroblasts
Head
In vitro methods and tests
Molecular
Optical and Plasma Physics
Physical Chemistry
Physics
Physics and Astronomy
Pretreatment
Quantum Information Technology
Quantum Physics
Radiation therapy
Regular Article
Repair
Spectroscopy/Spectrometry
Spintronics
Topical Issue: Dynamics of Systems at the Nanoscale
Tumors
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Summary:In order to improve patients’ post-treatment quality of life, a shift from surgery to non-surgical (chemo)radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of γ H2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of γ -rays. To better understand complex tumor behavior, three different cell type primocultures – CD90 − , CD90 + , and a mixed culture of these cells – were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV–HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance. Graphical abstract
ISSN:1434-6060
1434-6079
DOI:10.1140/epjd/e2017-80073-2