Detection of staphylococcal enterotoxin a by phage display mediated immuno-PCR method

A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phag...

Full description

Saved in:
Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2017-09, Vol.43 (5), p.540-543
Main Authors: Artykov, A. A., Fursova, K. K., Ryazantsev, D. Yu, Shchannikova, M. P., Loskutova, I. V., Shepelyakovskaya, A. O., Laman, A. G., Zavriev, S. K., Brovko, F. A.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Deoxyribonucleic acid
DNA
Life Sciences
Milk
Organic Chemistry
Phages
Polymerase chain reaction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phage display mediated immuno-PCR method is 100 pg/mL that is much lower than the level of toxic and allergic dose.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162017050028