Development of a Polymerase Chain Reaction System for the Detection of Dog and Cat Meat in Meat Mixtures and Animal Feed

ABSTRACT The identification of species origin of meat represents a considerable problem for food and animal feed analysis. In the present study a PCR‐mediated method for the detection of dog and cat meat was developed. For this the cytochrome b gene sequence of both species was analyzed by restricti...

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Bibliographic Details
Published in:Journal of food science 2003-06, Vol.68 (5), p.1757-1761
Main Authors: Abdulmawjood, A., Schönenbrücher, H., Bülte, M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
cat meat
dog meat
Enzymes
Feed and pet food industries
Feeds
Food industries
Food science
Fundamental and applied biological sciences. Psychology
Heat treating
Meat
Meat and meat product industries
PCR
RLFP
species-specific primer
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Summary:ABSTRACT The identification of species origin of meat represents a considerable problem for food and animal feed analysis. In the present study a PCR‐mediated method for the detection of dog and cat meat was developed. For this the cytochrome b gene sequence of both species was analyzed by restriction fragment length polymorphism (RFLP) analysis. The use of the restriction enzymes Alu I and Hae III yielded specific restriction profiles characteristic for each species. The meat of both species could additionally be differentiated with species‐specific oligonucleotide primers based on specific parts of the cytochrome b gene sequences characteristic for dog and cat. The use of these oligonucleotide primers allowed a direct identification of dog and cat meat in meat mixtures even after heat treatment.
ISSN:0022-1147
1750-3841
DOI:10.1111/j.1365-2621.2003.tb12324.x