Hydrolysis of Caseins By Extracts of Cynara Cardunculus Precipitated by Ammonium Sulfate

Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammo...

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Bibliographic Details
Published in:Journal of food science 2002-06, Vol.67 (5), p.1746-1751
Main Authors: Silva, S.V., Barros, R.M., Malcata, F.X.
Format: Article
Language:English
Subjects:
Agricultural and forest climatology and meteorology. Irrigation. Drainage
Agronomy. Soil science and plant productions
Ammonia
Biological and medical sciences
Enzymes
Food industries
Food science
FPLC
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Irrigation. Drainage
Milk and cheese industries. Ice creams
milk proteins
thistle
urea-PAGE
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Summary:Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant‐to‐proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.
ISSN:0022-1147
1750-3841
DOI:10.1111/j.1365-2621.2002.tb08717.x