Rapid methods for lipoxygenase assay in sweet corn

Potential methods for rapid assay of sweet corn lipoxygenase (LPO) were evaluated and compared by visual inspection. Both model system (partially purified) and vegetable (LPO extract) system studies were carried out. Model system studies indicated that LPO had maximum activity at pH 6.0 and 2.0 mM l...

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Bibliographic Details
Published in:Journal of food science 1997-07, Vol.62 (4), p.696-700
Main Authors: Romero, M.V. (University of California, Davis, CA.), Barrett, D.M
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANALYTICAL METHODS
Biological and medical sciences
Cereal and baking product industries
Corn
Enzymes
ENZYMIC ACTIVITY
EXPERIMENTACION
EXPERIMENTATION
Food industries
Food science
Fundamental and applied biological sciences. Psychology
LIPOXIGENASA
LIPOXYGENASE
MAIS
MAIZ
MAIZE
methylene blue
rapid assay
RAPID ASSAYS
sweet corn
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
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Summary:Potential methods for rapid assay of sweet corn lipoxygenase (LPO) were evaluated and compared by visual inspection. Both model system (partially purified) and vegetable (LPO extract) system studies were carried out. Model system studies indicated that LPO had maximum activity at pH 6.0 and 2.0 mM linoleic acid. Methylene blue bleaching (MBB), carotene bleaching (CB), and potassium iodide-starch (KI-S) methods were evaluated both spectrophotometrically and visually. Both MBB and KI-S methods were effective for the model system. For the vegetable LPO extract systems (sweet corn and green beans), the MBB method indicated positive results for both, the CB method was not effective for either, and the KI-S method was very effective for green beans but not for sweet corn
ISSN:0022-1147
1750-3841
DOI:10.1111/j.1365-2621.1997.tb15438.x