Licofelone Inhibits Proliferation of Rat Hepatoma Cells

Despite clinical treatments, hepatocellular carcinoma is one of the most common causes of cancer death. We tested whether licofelone, an inhibitor of both cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism, changes the survival rate and induces apoptosis in a hepatoma cell line...

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Bibliographic Details
Published in:Pakistan journal of zoology 2017-06, Vol.49 (3), p.811-817
Main Authors: Oztopcu-Vatan, Pinar, Kus, Gokhan, Inan, Emine, Korkut, Melek Gunindi, Kabadere, Selda, Uyar, Ruhi
Format: Article
Language:English
Subjects:
5-Fluorouracil
Apoptosis
Arachidonic acid
Cancer therapies
Cancer treatment
Care and treatment
Cell proliferation
Cell survival
Colorectal cancer
COX-2 inhibitors
Disease prevention
Drug dosages
Flow cytometry
Health aspects
Hepatocellular carcinoma
Hepatoma
Inflammation
Lipoxygenase
Liver cancer
Metabolism
Metabolites
Methods
Mortality
Prostaglandin endoperoxide synthase
Prostate
Rodents
Studies
Survival
Time dependence
Variance analysis
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Summary:Despite clinical treatments, hepatocellular carcinoma is one of the most common causes of cancer death. We tested whether licofelone, an inhibitor of both cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism, changes the survival rate and induces apoptosis in a hepatoma cell line (H4IIE). We determined in vitro survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey's multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h and 140 µM for 48 h applications. Used as a positive control, 250 µM 5-Fluorouracil decreased the cell survival by only 40 % after 48 h, but licofelone treatment with the same dose and time duration decreased the number of surviving cells by 87%.Treatments with 150, 200 and 250 µM licofelone caused early apoptotic cell rates of 4, 15 and 24 % for 24 h and 6, 13 and 24 % for 48 h, respectively. In addition, 150, 200 and 250 µM of 5-Fluorouracil resulted early apoptotic cell values of 13, 12, and 11 % for 24 h and 18, 22 and 16 % for 48 h. This study revealed that licofelone possesses dose and time dependent anti-proliferative and apoptotic properties on hepatoma cells.
ISSN:0030-9923
DOI:10.17582/journal.pjz/2017.49.3.811.817