Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

BACKGROUND Bacteriophage M13 is an Escherichia coli‐specific non‐lytic filamentous virus commonly used in applications ranging from antibody screening and nanomaterial construction to drug delivery, among others. In this tenor, alternative methods for the fractionation, recovery and partial purifica...

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Published in:Journal of chemical technology and biotechnology (1986) 2017-11, Vol.92 (11), p.2808-2816
Main Authors: González‐Mora, Alejandro, Ruiz‐Ruiz, Federico, Benavides, Jorge, Willson, Richard C, Rito‐Palomares, Marco
Format: Article
Language:English
Subjects:
aqueous two‐phase systems
bacteriophage M13
Binary systems
Drug delivery
Drug delivery systems
E coli
Extraction
Fermentation
Fractionation
ionic liquid
Ionic liquids
Nanomaterials
Particulates
partitioning
Phages
Phosphates
plaque forming assay, purification
Polyethylene glycol
Potassium
Potassium phosphate
Protein folding
Protein purification
Purification
Recovery
Salts
Systems analysis
Viruses
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Summary:BACKGROUND Bacteriophage M13 is an Escherichia coli‐specific non‐lytic filamentous virus commonly used in applications ranging from antibody screening and nanomaterial construction to drug delivery, among others. In this tenor, alternative methods for the fractionation, recovery and partial purification of phage particles are desired. In this work, the use of aqueous two‐phase systems (ATPS) was evaluated as an alternative method for the recovery of phage particles. RESULTS The partition behavior of M13 in PEG–salt and ionic liquid (IL)–salt ATPS was characterized using a pre‐purified feedstock. In PEG‐salt ATPS, M13 was preferentially partitioned to the interface. In IL ATPS, however, M13 showed a high‐top phase preference with recovery yields above 65%. Selected systems were tested for the extraction of M13 from a crude fermentation broth. From crude broth, a PEG 400‐potassium phosphate system with volume ratio (VR) of 1 and 25% w/w tie line length (TLL) gave the best M13 top phase recovery (83%) and purification fold (18.2) in terms of total protein concentration. CONCLUSIONS The results presented here demonstrate the practical application of ATPS as an efficient process for the primary recovery and partial purification of M13 and represent the first study of the extraction of viral particles directly from a crude broth as well as the use of IL‐Salt ATPS. © 2017 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.5359