Low molecular chitosan–(poly)nitroxides: Synthesis and evaluation as antioxidants on free radical-induced erythrocyte hemolysis

Water-soluble chitosan-(poly)nitroxides (CPNs), differing in molecular weight of the saccharide backbone and the nature of nitroxide radical, were obtained and characterized. Fractions of modified glucosamines were in the range 0.15–0.28. The dependence of delay time of 2,2-azobis-2-methylpropanimid...

Full description

Saved in:
Bibliographic Details
Published in:Reactive & functional polymers 2017-02, Vol.111, p.53-59
Main Authors: Sen', Vasily D., Sokolova, Ekaterina M., Neshev, Nikolai I., Kulikov, Alexander V., Pliss, Evgeny M.
Format: Article
Language:English
Subjects:
Antioxidant capacity
Antioxidants
Binding
Cations
Chemical synthesis
Chitosan
Delay
Electron paramagnetic resonance
EPR spectroscopy
Erythrocyte hemolysis
Erythrocytes
Hemolysis
Low-molecular-weight-chitosans
Molecular weight
Nitroxide coupling
Oxidation
Piperidine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Water-soluble chitosan-(poly)nitroxides (CPNs), differing in molecular weight of the saccharide backbone and the nature of nitroxide radical, were obtained and characterized. Fractions of modified glucosamines were in the range 0.15–0.28. The dependence of delay time of 2,2-azobis-2-methylpropanimidamide dihydrochloride (AAPH)-induced erythrocyte hemolysis on the concentration of CPNs and data of electron paramagnetic resonance (EPR) spectra indicate their binding to the cell membrane. Because of this binding, CPNs demonstrate identical delay times of hemolysis at concentrations ~100 times lower than structurally similar low molecular nitroxides. Due to easier oxidation of piperidine nitroxide by radicals RO2 to oxoammonium cations, CPNs with attached 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) more effectively inhibit hemolysis than their counterparts with 2,2,5,5-tetramethylpyrroline-1-oxyl. Recycling of nitroxides, presumably, take place through the reaction of oxoammonium cations with common biological reductants. Taken together, our study suggests that CPNs are of interest both as biologically active compounds and as research instruments.
ISSN:1381-5148
1873-166X
DOI:10.1016/j.reactfunctpolym.2016.12.006