The [alpha]-galactosyl derivatives of ganglioside GD1b are essential for the organization of lipid rafts in RBL-2H3 mast cells

Gangliosides are complex glycosphingolipids that are important in many biological processes. The present study investigated the role of gangliosides in the organization of lipid rafts in RBL-2H3 mast cells and in the modulation of mast cell degranulation via Fc[varepsilon]RI. The role of ganglioside...

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Bibliographic Details
Published in:Experimental cell research 2008-08, Vol.314 (13), p.2515
Main Authors: Silveira e Souza, Adriana Maria Mariano, Mazucato, Vivian Marino, de Castro, Rodrigo Orlandini, Matioli, Fabiana, Ciancaglini, Pietro, de Paiva Paulino, Tony, Jamur, Maria Célia, Oliver, Constance
Format: Article
Language:English
Subjects:
Cellular biology
Lipids
Mutation
Signal transduction
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Summary:Gangliosides are complex glycosphingolipids that are important in many biological processes. The present study investigated the role of gangliosides in the organization of lipid rafts in RBL-2H3 mast cells and in the modulation of mast cell degranulation via Fc[varepsilon]RI. The role of gangliosides was examined using two ganglioside deficient cell lines (B6A4A2III-E5 and B6A4C1III-D1) as well as the parent cell line (RBL-2H3). All three cell lines examined express Fc[varepsilon]RI, Lyn, Syk and LAT. However, only in RBL-2H3 cells were Fc[varepsilon]RI, LAT and [alpha]-galactosyl derivatives of ganglioside GD1b mobilized to lipid raft domains following Fc[varepsilon]RI stimulation. The inhibition of glycosphingolipid synthesis in RBL-2H3 cells also resulted in a decrease in the release of [beta]-hexosaminidase activity after Fc[varepsilon]RI activation. The two mutant cell lines have a reduced release of [beta]-hexosaminidase activity after Fc[varepsilon]RI stimulation, but not after exposure to calcium ionophore. These results indicate that the [alpha]-galactosyl derivatives of ganglioside GD1b are important in the initial events of Fc[varepsilon]RI signaling upstream of Ca2+ influx. Since the initial signaling events occur in lipid rafts and in the mutant cell lines the rafts are disorganized, these results also suggest that these gangliosides contribute to the correct assembly of lipid rafts and are essential for mast cell activation via Fc[varepsilon]RI. [PUBLICATION ABSTRACT]
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2008.05.014