Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents

• Published in: Process biochemistry (1991) 2017-05, Vol.56, p.81-89
• Main Authors: Cieh, Ng Lin, Sulaiman, Safwan, Mokhtar, Mohd Noriznan, Naim, Mohd Nazli
• Format: Article
• Language: English
• Subjects: Antimicrobial agents; Binding; Bleached kenaf microfiber; Bleaching; Coupling agents; Covalent immobilization; Cyclodextrin; Cyclodextrin glucanotransferase (CGTase); Deactivation; Enzymatic activity; Enzyme activity; Enzymes; Ethylenediamine; Glutaraldehyde; Immobilization; Kenaf; Ligand; Microorganisms; Shelf life; Spacer arm; Stability analysis; Storage; Storage stability; Strong interactions (field theory); Thermal stability
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2017.02.025

[Display omitted] •Kenaf microfiber has potential as enzyme immobilization support.•Different spacer arms and ligands affect the properties of immobilized CGTase.•Thermal stability of immobilized CGTase is better than its free form.•Lower deactivation constant reflected to higher stability of immobilized CGTase. Enzyme immobilization via covalent binding provides a strong interaction between enzyme and support material. In this study, the effect of different coupling agents (spacer arms and ligands) in cyclodextrin glucanotransferase (CGTase) immobilization on bleached kenaf microfiber as a support matrix was investigated. The immobilized CGTase properties such as storage stability, thermal stability and reusability were evaluated. Immobilized CGTases on microfiber resulted in 0.162–0.24U/mg-fiber when 55.6U/mL of CGTase activity was initially added during the immobilization. The highest storage stability (60°C) was shown by CGTase that was immobilized with ethylenediamine and o-phthalaldehyde, whereby 60% of its activity remained after 15days. Its high stability was also confirmed by the lowest deactivation constant, kd that was obtained at 25°C (0.0161day−1) and 60°C (0.0361day−1). The CGTase immobilized using ethylenediamine and glutaraldehyde has shown the best retention of enzyme activity up to 72.72% after 12 cycles of batch reaction. The results indicate that kenaf microfiber has potential to be applied as a support for enzyme immobilization and its enzymatic properties were affected by the coupling agents.