Modification of Titanium Surfaces via Surface-initiated Atom Transfer Radical Polymerization to Graft PEG-RGD Polymer Brushes to Inhibit Bacterial Adhesion and Promote Osteoblast Cell Attachment

• Published in: Journal of Wuhan University of Technology. Materials science edition 2017-10, Vol.32 (5), p.1225-1231
• Main Authors: 陈琦, LIU Di, GONG Yuejiao, XIAO Qun, 李志安
• Format: Article
• Language: English
• Subjects: Adhesion; Antiinfectives and antibacterials; Attachment; Bacteria; Biocompatibility; Biomaterials; Brushes; Chemistry and Materials Science; Clinical medicine; Contact angle; Coupling agents; Ethylene glycol; Fluorescence; Fouling; Hydrophobicity; Materials Science; Microscopy; Polymerization; Surgical implants; Titanium; X ray photoelectron spectroscopy; 原子转移自由基聚合; 引发; 成骨细胞; 细菌粘附; 聚合改性; 聚合物刷; 钛表面; 附着能力
• ISSN: 1000-2413; 1993-0437
• DOI: 10.1007/s11595-017-1735-2

Implant-related infection is one of the key concerns in clinical medicine, so the modification of titanium to inhibit bacterial adhesion and support osteoblast cell attachment is important. In this article, two strategies were used to examine the above effects. First, modification of titanium via surface-initiated atom transfer radical polymerization（ATRP） was performed. The surface of the titanium was activated initially by a silane coupling agent. Well-defined polymer brushes of poly（ethylene glycol） methacrylate were successfully tethered on the silane-coupled titanium surface to form hydration shell to examine the anti-fouling effect. Second, functionalization of the Ti-PEG surface with RGD was performed to examine the anti-bacterial adhesion and osteoblast cell attachment ability. The chemical composition of modified titanium surfaces was characterized by X-ray photoelectron spectroscopy（XPS）. Changes in surface hydrophilicity and hydrophobicity were characterized by static water contact angle measurements. Results indicated that PEG-RGD brushes were successfully tethered on the titanium surface. And anti-bacterial adhesion ability and osteoblast cell attachment ability were confirmed by fluorescence microscopy and scanning electron microscopy. Results indicated that PEG can inhibit both bacterial adhesion and osteoblast cell attachment, while PEG-RGD brushes can not only inhibit bacterial adhesion but also promote osteoblast cell attachment.