Multiclass mycotoxin analysis in edible oils using a simple solvent extraction method and liquid chromatography with tandem mass spectrometry

• Published in: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2017-11, Vol.34 (11), p.2011-2022
• Main Authors: Eom, Taeyong, Cho, Hyun-Deok, Kim, Junghyun, Park, Mihee, An, Jinyoung, Kim, Moosung, Kim, Sheen-Hee, Han, Sang Beom
• Format: Article
• Language: English
• Subjects: Acetonitrile; Aflatoxins; Chromatography; Chromatography, Liquid; Contamination; Corn oil; Deoxynivalenol; edible oil; Edible oils; Fats; Formic acid; Fumonisins; Ionization; Ions; LC-MS/MS; Linearity; Liquid chromatography; Liquid Phase Microextraction; Liquid-liquid extraction; Mass spectrometry; Mass spectroscopy; multiclass mycotoxin; Mycotoxins; Mycotoxins - analysis; n-Hexane; Ochratoxin A; Oils & fats; Plant Oils - chemistry; Plants, Edible - chemistry; Polarity; Rice bran oil; Sample preparation; Scientific imaging; Simultaneous analysis; Solvent extraction; Solvents; Solvents - chemistry; Soybean oil; Spectroscopy; Supply & demand; Switching (polarity); Tandem Mass Spectrometry; Zearalenone
• ISSN: 1944-0049; 1944-0057
• DOI: 10.1080/19440049.2017.1363416

A simple and rapid method for the simultaneous determination of 11 mycotoxins - aflatoxins B 1 , B 2 , G 1 and G 2 ; fumonisins B 1 , B 2 and B 3 ; ochratoxin A; zearalenone; deoxynivalenol; and T-2 toxin - in edible oils was established using liquid chromatography tandem mass spectrometry (LC-MS/MS). In this study, QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe), QuEChERS with dispersive liquid-liquid microextraction, and solvent extraction were examined for sample preparation. Among these methods, solvent extraction with a mixture of formic acid/acetonitrile (5/95, v/v) successfully extracted all target mycotoxins. Subsequently, a defatting process using n-hexane was employed to remove the fats present in the edible oil samples. Mass spectrometry was carried out using electrospray ionisation in polarity switching mode with multiple reaction monitoring. The developed LC-MS/MS method was validated by assessing the specificity, linearity, recovery, limit of quantification (LOQ), accuracy and precision with reference to Commission Regulation (EC) 401/2006. Mycotoxin recoveries of 51.6-82.8% were achieved in addition to LOQs ranging from 0.025 ng/g to 1 ng/g. The edible oils proved to be relatively uncomplicated matrices and the developed method was applied to 9 edible oil samples, including soybean oil, corn oil and rice bran oil, to evaluate potential mycotoxin contamination. The levels of detection were significantly lower than the international regulatory standards. Therefore, we expect that our developed method, based on simple, two-step sample preparation process, will be suitable for the large-scale screening of mycotoxin contamination in edible oils.