A modified protocol for high-quality DNA extraction from seeds rich in secondary compounds

High-quality DNA is a prerequisite for a range of molecular biology experiments and thus DNA extraction is one of the most important steps for several downstream experiments. DNA isolation directly from seeds could save time and effort, particularly for large-scale experiments where growing and main...

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Bibliographic Details
Published in:Journal of crop improvement 2017-09, Vol.31 (5), p.637-647
Main Authors: Sudan, Jebi, Raina, Meenakshi, Singh, Ravinder, Mustafiz, Ananda, Kumari, Sumita
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
DNA purity
Experiments
Extraction
Genotypes
Glycerol
Molecular biology
Mucilage
Oils & fats
Polyphenols
Polysaccharides
Reagents
Saccharides
Seeds
SGS buffer
Sodium
Sodium dodecyl sulfate
Sodium lauryl sulfate
Sucrose
Sugar
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Summary:High-quality DNA is a prerequisite for a range of molecular biology experiments and thus DNA extraction is one of the most important steps for several downstream experiments. DNA isolation directly from seeds could save time and effort, particularly for large-scale experiments where growing and maintaining multitude of genotypes in parallel is cumbersome. However, seeds often contain polysaccharides, polyphenols, mucilage, oils, etc., which cause DNA extraction from seeds difficult and sometimes a research limiting step. In the present study, we have considered many previous protocols and optimized these methods to devise a general method for extraction of DNA from the seeds of diverse plant genera. The new SGS buffer (sucrose, glycerol and sodium dodecyl sulphate) was used to extract intact DNA from seeds with yield and quality better than in the previous protocols. Moreover, the proposed protocol is devoid of hazardous and expensive reagents and can be scaled up.
ISSN:1542-7528
1542-7536
DOI:10.1080/15427528.2017.1345028