Lactic Acid Bacteria Combinations for Wheat Sourdough Preparation and Their Influence on Wheat Bread Quality and Acrylamide Formation

• Published in: Journal of food science 2017-10, Vol.82 (10), p.2371-2378
• Main Authors: Bartkiene, Elena, Bartkevics, Vadims, Krungleviciute, Vita, Pugajeva, Iveta, Zadeike, Daiva, Juodeikiene, Grazina
• Format: Article
• Language: English
• Subjects: Acceptability; Acidification; Acrylamide; Acrylamide - analysis; Acrylamide - metabolism; Agricultural production; Arabinose; Bacteria; Bread; Bread - analysis; Bread - microbiology; Carbohydrate metabolism; Carbohydrates; D-Galactose; D-Ribose; Enterococcus pseudoavium; Fermentation; Food Handling; Food Microbiology; Fructose; Galactose; Galactose - metabolism; Lactic acid; Lactic Acid - metabolism; Lactic acid bacteria; Lactobacillales - classification; Lactobacillales - genetics; Lactobacillales - isolation & purification; Lactobacillales - metabolism; Lactobacillus brevis; Lactobacillus curvatus; Lactobacillus plantarum; Leuconostoc mesenteroides; Maltose; Metabolism; Moisture content; Pediococcus pentosaceus; Porosity; quality; Sourdough; Specific volume; Strains (organisms); Triticum; Triticum - chemistry; Triticum - metabolism; Triticum - microbiology; Wheat; wheat bread
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.13858

Different lactic acid bacteria (LAB) from spontaneous wheat sourdough were isolated, identified, and characterized by their growth, acidification rate, and carbohydrate metabolism. The combinations of isolated LAB (Pediococcus pentosaceus LUHS183 and Leuconostoc mesenteroides LUHS242, P. pentosaceus LUHS183 and Lactobacillus brevis LUHS173, P. pentosaceus LUHS183 and Enterococcus pseudoavium LUHS 234, P. pentosaceus LUHS183 and Lactobacillus curvatus LUHS51, Lactobacillus plantarum LUHS135 and L. curvatus LUHS51, L. plantarum LUHS135 and P. pentosaceus LUHS183) were used for wheat sourdough production, and the effects of LAB fermentation in sourdoughs on wheat bread quality parameters and acrylamide formation were evaluated. All of the tested strains (except E. pseudoavium LUHS 234) were able to ferment l‐arabinose, d‐ribose, d‐galactose, d‐fructose, and d‐maltose and showed high tolerance to acidic conditions. The highest overall acceptability (135.8 ± 5.5 mm) was found in the bread produced with L. plantarum and P. pentosaceus sourdough. This group of bread also showed the highest shape coefficient (2.59 ± 0.02), the highest specific volume (3.40 ± 0.03 cm3/g), the highest porosity (76.6 ± 0.3%), and the highest moisture content (33.7%). Selected sourdoughs reduced acrylamide content in bread samples by 29.5% (sourdough prepared with P. pentosaceus and L. mesenteroides) to 67.2% (sourdough prepared with P. pentosaceus and L. curvatus). These cultures potentially can be used to reduce acrylamide in breads. Practical Application The data of this study have practical applications. L. plantarum and P. pentosaceus sourdoughs increases overall acceptability, specific volume, and porosity of wheat bread. Besides the fact that sourdoughs produced by using combinations of selected LAB strains improved the quality parameters of bread, fermentation with prepared sourdoughs also reduced the acrylamide content in wheat bread samples by 29.5% (sourdough prepared with P. pentosaceus and L. mesenteroides) to 67.2% (sourdough prepared with P. pentosaceus and L. curvatus).