A non-cytotoxic pseudo peptide KL1 with potent antioxidant activity

The reaction mixture containing FeCİ3 (100 gM), EDTA (104 gM), H2O2 (1 mM) and 2-deoxy- D-ribose (2.8 mM) were mixed with or without KL1 at various concentrations (10 gM to 100 gM) in 1 ml final reaction volume made with potassium phosphate buffer (20 mM pH 7.4) and incubated for one hour at 37°C. B...

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Bibliographic Details
Published in:Asian Journal of Research in Chemistry 2017-03, Vol.10 (2), p.127
Main Authors: Gangadhara, MM, Dinesha, R, Murari, Satish Kumar
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Apoptosis
Cytotoxicity
Lymphocytes
Methods
Oxidation
Oxidative stress
Peptides
Prevention
Proteins
Statistical analysis
Vitamins
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Description
Summary:The reaction mixture containing FeCİ3 (100 gM), EDTA (104 gM), H2O2 (1 mM) and 2-deoxy- D-ribose (2.8 mM) were mixed with or without KL1 at various concentrations (10 gM to 100 gM) in 1 ml final reaction volume made with potassium phosphate buffer (20 mM pH 7.4) and incubated for one hour at 37°C. BHA and BHT (400gM) were used as positive control. [...]the reaction mixture was cooled on ice and centrifuged at 5000 rpm for 15 min. Prevention of H2O2 induced cell death by KL1: Human peripheral lymphocytes were isolated from freshly drawn human blood and cell viability was monitored by Tryphan blue dye exclusion method according to H.J. Phillips, (1973) method [9]. Mylarappa B Ningappa, Dinesha Ramadas, Dinesha Nanjegowda, Kiruthika Balasubramanian, Khusdeep Chahal, Sachin Patil and Leela Srinivas Cytoprotective Properties of Antioxidant Protein from Curry leaves (Murraya koenigii L.) against Oxidative Stress Induced Damage in Human Erythrocytes, Medicinal chemistry, 6(2): 2016;075-080.
ISSN:0974-4169
0974-4150
DOI:10.5958/0974-4150.2017.00020.7