Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.)

Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid geno...

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Bibliographic Details
Published in:Annals of botany 1999-05, Vol.83 (5), p.535-541
Main Authors: NOUZOVÁ, MARCELA, KUBALÁKOVÁ, MARIE, DOLEŽELOVÁ, MARIE, KOBLÍŽKOVÁ, ANDREA, NEUMANN, PAVEL, DOLEŽEL, JAROSLAV, MACAS, JIŘÍ
Format: Article
Language:English
Subjects:
Chromosomes
copy number
DNA
DNA probes
field bean
FISH
genbank/aj011708
genbank/aj011709
genbank/aj011710
genbank/aj011711
genbank/aj222863
genbank/aj222864
genbank/aj222865
genbank/aj222866
genbank/aj222867
genbank/aj222868
genes
Genetic hybridization
genetic techniques and protocols
genome organization
Genomes
Genomics
In situ hybridization
loci
mitosis
molecular sequence data
Nucleic acid hybridization
nucleotide sequences
Plants
PRINS
repeated DNA sequences
Repetitive sequences
restriction mapping
Vicia faba
Vicia faba, field bean, repeated DNA sequences, FISH, PRINS, genome organization, copy number
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Description
Summary:Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid genome. Southern blot andin situhybridization revealed that four of five newly isolated repeats were dispersed in theV. fabagenome. One of the repeats (TIII15) showed tandem organization with several major hybridization spots on mitotic chromosomesin situ.These sites were distributed in euchromatic as well as in heterochromatic chromosomal regions, and in several loci they were simultaneously localized with previously describedFokI repeated elements. The sequence of TIII15 comprises four 26–27 bp subrepeats, but shares no homology toFokI elements which have similar sequence organization. All newly described sequences were highly specific forV. faba,with little or no hybridization to DNA of otherViciaspecies, and no hybridization to DNA of other legumes tested.
ISSN:0305-7364
1095-8290
DOI:10.1006/anbo.1999.0853