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Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.)
Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid geno...
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| Published in: | Annals of botany 1999-05, Vol.83 (5), p.535-541 |
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| container_end_page | 541 |
| container_issue | 5 |
| container_start_page | 535 |
| container_title | Annals of botany |
| container_volume | 83 |
| creator | NOUZOVÁ, MARCELA KUBALÁKOVÁ, MARIE DOLEŽELOVÁ, MARIE KOBLÍŽKOVÁ, ANDREA NEUMANN, PAVEL DOLEŽEL, JAROSLAV MACAS, JIŘÍ |
| description | Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid genome. Southern blot andin situhybridization revealed that four of five newly isolated repeats were dispersed in theV. fabagenome. One of the repeats (TIII15) showed tandem organization with several major hybridization spots on mitotic chromosomesin situ.These sites were distributed in euchromatic as well as in heterochromatic chromosomal regions, and in several loci they were simultaneously localized with previously describedFokI repeated elements. The sequence of TIII15 comprises four 26–27 bp subrepeats, but shares no homology toFokI elements which have similar sequence organization. All newly described sequences were highly specific forV. faba,with little or no hybridization to DNA of otherViciaspecies, and no hybridization to DNA of other legumes tested. |
| doi_str_mv | 10.1006/anbo.1999.0853 |
| format | article |
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The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid genome. Southern blot andin situhybridization revealed that four of five newly isolated repeats were dispersed in theV. fabagenome. One of the repeats (TIII15) showed tandem organization with several major hybridization spots on mitotic chromosomesin situ.These sites were distributed in euchromatic as well as in heterochromatic chromosomal regions, and in several loci they were simultaneously localized with previously describedFokI repeated elements. The sequence of TIII15 comprises four 26–27 bp subrepeats, but shares no homology toFokI elements which have similar sequence organization. All newly described sequences were highly specific forV. faba,with little or no hybridization to DNA of otherViciaspecies, and no hybridization to DNA of other legumes tested.</description><identifier>ISSN: 0305-7364</identifier><identifier>EISSN: 1095-8290</identifier><identifier>DOI: 10.1006/anbo.1999.0853</identifier><language>eng</language><publisher>Oxford: Elsevier Science Ltd</publisher><subject>Chromosomes ; copy number ; DNA ; DNA probes ; field bean ; FISH ; genbank/aj011708 ; genbank/aj011709 ; genbank/aj011710 ; genbank/aj011711 ; genbank/aj222863 ; genbank/aj222864 ; genbank/aj222865 ; genbank/aj222866 ; genbank/aj222867 ; genbank/aj222868 ; genes ; Genetic hybridization ; genetic techniques and protocols ; genome organization ; Genomes ; Genomics ; In situ hybridization ; loci ; mitosis ; molecular sequence data ; Nucleic acid hybridization ; nucleotide sequences ; Plants ; PRINS ; repeated DNA sequences ; Repetitive sequences ; restriction mapping ; Vicia faba ; Vicia faba, field bean, repeated DNA sequences, FISH, PRINS, genome organization, copy number</subject><ispartof>Annals of botany, 1999-05, Vol.83 (5), p.535-541</ispartof><rights>1999 Annals of Botany Company</rights><rights>Copyright Oxford University Press(England) May 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-c4160e561ebe1453ee5fad5cccd38ef46530ac2cc68765175d6cc74305c771523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42765983$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42765983$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids></links><search><creatorcontrib>NOUZOVÁ, MARCELA</creatorcontrib><creatorcontrib>KUBALÁKOVÁ, MARIE</creatorcontrib><creatorcontrib>DOLEŽELOVÁ, MARIE</creatorcontrib><creatorcontrib>KOBLÍŽKOVÁ, ANDREA</creatorcontrib><creatorcontrib>NEUMANN, PAVEL</creatorcontrib><creatorcontrib>DOLEŽEL, JAROSLAV</creatorcontrib><creatorcontrib>MACAS, JIŘÍ</creatorcontrib><title>Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.)</title><title>Annals of botany</title><addtitle>Ann Bot</addtitle><description>Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid genome. Southern blot andin situhybridization revealed that four of five newly isolated repeats were dispersed in theV. fabagenome. One of the repeats (TIII15) showed tandem organization with several major hybridization spots on mitotic chromosomesin situ.These sites were distributed in euchromatic as well as in heterochromatic chromosomal regions, and in several loci they were simultaneously localized with previously describedFokI repeated elements. The sequence of TIII15 comprises four 26–27 bp subrepeats, but shares no homology toFokI elements which have similar sequence organization. All newly described sequences were highly specific forV. faba,with little or no hybridization to DNA of otherViciaspecies, and no hybridization to DNA of other legumes tested.</description><subject>Chromosomes</subject><subject>copy number</subject><subject>DNA</subject><subject>DNA probes</subject><subject>field bean</subject><subject>FISH</subject><subject>genbank/aj011708</subject><subject>genbank/aj011709</subject><subject>genbank/aj011710</subject><subject>genbank/aj011711</subject><subject>genbank/aj222863</subject><subject>genbank/aj222864</subject><subject>genbank/aj222865</subject><subject>genbank/aj222866</subject><subject>genbank/aj222867</subject><subject>genbank/aj222868</subject><subject>genes</subject><subject>Genetic hybridization</subject><subject>genetic techniques and protocols</subject><subject>genome organization</subject><subject>Genomes</subject><subject>Genomics</subject><subject>In situ hybridization</subject><subject>loci</subject><subject>mitosis</subject><subject>molecular sequence data</subject><subject>Nucleic acid hybridization</subject><subject>nucleotide sequences</subject><subject>Plants</subject><subject>PRINS</subject><subject>repeated DNA sequences</subject><subject>Repetitive sequences</subject><subject>restriction mapping</subject><subject>Vicia faba</subject><subject>Vicia faba, field bean, repeated DNA sequences, FISH, PRINS, genome organization, copy number</subject><issn>0305-7364</issn><issn>1095-8290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNp1kM1vFCEYh0mjSdfqtTcj8WQPM8IwwHDUtR8mmzW2bmN6ISzzTmXdwgr0w_71ZTKmNy9w-D0v_N4HoUNKakqI-Gj8OtRUKVWTjrM9NKNE8aprFHmBZoQRXkkm2n30KqUNIaQRis7Qar4N3vlrbHyP579MNDZDdI8mu-BxGPAS7vE57CC77O4AX8CfW_AWEnYenzjY9vgzGI8_XDrrDB7M2izqo9fo5WC2Cd78uw_Q6uT4x_ysWnw7_Tr_tKhsy2QuJxUEuKCwBtpyBsAH03Nrbc86GFrBGTG2sVZ0UnAqeS-slW1ZxUpJecMO0Pvp3V0MpVfKehNuoy9faqo4aSWlqkD1BNkYUoow6F10Nyb-1ZTo0ZwezenRnB7NlYG308Am5RCf6bYpJVQ35tWUu5Th4Tk38bcWkkmuz35eaXF-9UV-X57qZeHfTfxggjbX0SW9umgIZaRRpWYnCtFNBBRZdw6iTtaNmnsXwWbdB_e_sk_J7pVz</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>NOUZOVÁ, MARCELA</creator><creator>KUBALÁKOVÁ, MARIE</creator><creator>DOLEŽELOVÁ, MARIE</creator><creator>KOBLÍŽKOVÁ, ANDREA</creator><creator>NEUMANN, PAVEL</creator><creator>DOLEŽEL, JAROSLAV</creator><creator>MACAS, JIŘÍ</creator><general>Elsevier Science Ltd</general><general>Oxford University Press</general><general>Academic Press Limited</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19990501</creationdate><title>Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.)</title><author>NOUZOVÁ, MARCELA ; KUBALÁKOVÁ, MARIE ; DOLEŽELOVÁ, MARIE ; KOBLÍŽKOVÁ, ANDREA ; NEUMANN, PAVEL ; DOLEŽEL, JAROSLAV ; MACAS, JIŘÍ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-c4160e561ebe1453ee5fad5cccd38ef46530ac2cc68765175d6cc74305c771523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Chromosomes</topic><topic>copy number</topic><topic>DNA</topic><topic>DNA probes</topic><topic>field bean</topic><topic>FISH</topic><topic>genbank/aj011708</topic><topic>genbank/aj011709</topic><topic>genbank/aj011710</topic><topic>genbank/aj011711</topic><topic>genbank/aj222863</topic><topic>genbank/aj222864</topic><topic>genbank/aj222865</topic><topic>genbank/aj222866</topic><topic>genbank/aj222867</topic><topic>genbank/aj222868</topic><topic>genes</topic><topic>Genetic hybridization</topic><topic>genetic techniques and protocols</topic><topic>genome organization</topic><topic>Genomes</topic><topic>Genomics</topic><topic>In situ hybridization</topic><topic>loci</topic><topic>mitosis</topic><topic>molecular sequence data</topic><topic>Nucleic acid hybridization</topic><topic>nucleotide sequences</topic><topic>Plants</topic><topic>PRINS</topic><topic>repeated DNA sequences</topic><topic>Repetitive sequences</topic><topic>restriction mapping</topic><topic>Vicia faba</topic><topic>Vicia faba, field bean, repeated DNA sequences, FISH, PRINS, genome organization, copy number</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NOUZOVÁ, MARCELA</creatorcontrib><creatorcontrib>KUBALÁKOVÁ, MARIE</creatorcontrib><creatorcontrib>DOLEŽELOVÁ, MARIE</creatorcontrib><creatorcontrib>KOBLÍŽKOVÁ, ANDREA</creatorcontrib><creatorcontrib>NEUMANN, PAVEL</creatorcontrib><creatorcontrib>DOLEŽEL, JAROSLAV</creatorcontrib><creatorcontrib>MACAS, JIŘÍ</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Annals of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NOUZOVÁ, MARCELA</au><au>KUBALÁKOVÁ, MARIE</au><au>DOLEŽELOVÁ, MARIE</au><au>KOBLÍŽKOVÁ, ANDREA</au><au>NEUMANN, PAVEL</au><au>DOLEŽEL, JAROSLAV</au><au>MACAS, JIŘÍ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.)</atitle><jtitle>Annals of botany</jtitle><addtitle>Ann Bot</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>83</volume><issue>5</issue><spage>535</spage><epage>541</epage><pages>535-541</pages><issn>0305-7364</issn><eissn>1095-8290</eissn><abstract>Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid genome. Southern blot andin situhybridization revealed that four of five newly isolated repeats were dispersed in theV. fabagenome. One of the repeats (TIII15) showed tandem organization with several major hybridization spots on mitotic chromosomesin situ.These sites were distributed in euchromatic as well as in heterochromatic chromosomal regions, and in several loci they were simultaneously localized with previously describedFokI repeated elements. The sequence of TIII15 comprises four 26–27 bp subrepeats, but shares no homology toFokI elements which have similar sequence organization. All newly described sequences were highly specific forV. faba,with little or no hybridization to DNA of otherViciaspecies, and no hybridization to DNA of other legumes tested.</abstract><cop>Oxford</cop><pub>Elsevier Science Ltd</pub><doi>10.1006/anbo.1999.0853</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0305-7364 |
| ispartof | Annals of botany, 1999-05, Vol.83 (5), p.535-541 |
| issn | 0305-7364 1095-8290 |
| language | eng |
| recordid | cdi_proquest_journals_195047119 |
| source | JSTOR Archival Journals and Primary Sources Collection; Oxford Journals Online; PubMed Central |
| subjects | Chromosomes copy number DNA DNA probes field bean FISH genbank/aj011708 genbank/aj011709 genbank/aj011710 genbank/aj011711 genbank/aj222863 genbank/aj222864 genbank/aj222865 genbank/aj222866 genbank/aj222867 genbank/aj222868 genes Genetic hybridization genetic techniques and protocols genome organization Genomes Genomics In situ hybridization loci mitosis molecular sequence data Nucleic acid hybridization nucleotide sequences Plants PRINS repeated DNA sequences Repetitive sequences restriction mapping Vicia faba Vicia faba, field bean, repeated DNA sequences, FISH, PRINS, genome organization, copy number |
| title | Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.) |
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