A Study of Genetic Variation and Relationships within the Bamboo Subtribe Bambusinae using Amplified Fragment Length Polymorphism

Taxonomic and systematic studies of the woody bamboos are traditionally based on floral morphology, which can cause problems in identification due to the lack of, or infrequent, flowering. Limited studies have been conducted using molecular techniques to overcome this problem. In this study, we used...

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Bibliographic Details
Published in:Annals of botany 2000-05, Vol.85 (5), p.607-612
Main Authors: Loh, Jin Phang, Kiew, Ruth, Set, Ohn, Gan, Leong Huat, Gan, Yik-Yuen
Format: Article
Language:English
Subjects:
AFLP
AFLP markers
Bamboo
Bamboo, Bambusinae, Bambusa, Gigantochloa, Dendrocalamus, Thyrsostachys, AFLP, diversity, AFLP markers
Bamboos
Bambusa
Bambusinae
Biological taxonomies
Botanical gardens
Dendrocalamus
diversity
DNA
Genera
Genetic diversity
Genetic variation
Gigantochloa
Molecular genetics
Plant morphology
Plants
Thyrsostachys
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Summary:Taxonomic and systematic studies of the woody bamboos are traditionally based on floral morphology, which can cause problems in identification due to the lack of, or infrequent, flowering. Limited studies have been conducted using molecular techniques to overcome this problem. In this study, we used amplified fragment length polymorphisms (AFLPs) to conduct a study of four genera of bamboos (Bambusa, Dendrocalamus, Gigantochloa andThyrsostachys ) in the subtribe Bambusinae. AFLP analysis using eight primer combinations was carried out on 15 species of bamboo. Results showed that AFLPs distinguish the different species by their unique banding patterns. Unique AFLPs were detected in 13 of the 15 species examined. The six Bambusa species examined separated into two clusters. The sixGigantochloa species studied formed a discrete cluster diverging from one of the Bambusa clusters, whileThyrsostachys was less similar to the Bambusa clusters. The similarity index between B. lako and G. atroviolacea was the highest, suggesting that B. lako is more appropriately included within the genusGigantochloa rather than the genus Bambusa. The two Dendrocalamus species examined were very different with D. brandisii clustering within one of the Bambusa clusters and D. giganteus appearing as a very distant species. These results support the contention that critical study of the genus Dendrocalamus is required. The use of AFLPs for identification of particular bamboo species, as well as for the study of relationships within the subtribe, will be useful for industrial purposes and for systematic studies.
ISSN:0305-7364
1095-8290
DOI:10.1006/anbo.2000.1109