Structure elucidation of degradation products of the antibiotic amoxicillin with ion trap MSn and accurate mass determination by ESI TOF

Today, it is necessary to identify relevant compounds appearing in discovery and development of new drug substances in the pharmaceutical industry. For that purpose, the measurement of accurate molecular mass and empirical formula calculation is very important for structure elucidation in addition t...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2005-10, Vol.16 (10), p.1670-1676
Main Authors: NÄGELE, Edgar, MORITZ, Ralf
Format: Article
Language:English
Subjects:
Amoxicillin
Antibacterial agents
Antibiotics
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Chromatography
Degradation
Empirical analysis
Fragments
General aspects
Identification methods
Ionization
Ions
Mass spectrometry
Medical sciences
Molecular ions
Pharmacology. Drug treatments
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Summary:Today, it is necessary to identify relevant compounds appearing in discovery and development of new drug substances in the pharmaceutical industry. For that purpose, the measurement of accurate molecular mass and empirical formula calculation is very important for structure elucidation in addition to other available analytical methods. In this work, the identification and confirmation of degradation products in a finished dosage form of the antibiotic drug amoxicillin obtained under stress conditions will be demonstrated. Structure elucidation is performed utilizing liquid chromatography (LC) ion trap MS/MS and MS^sup 3^ together with accurate mass measurement of the molecular ions and of the collision induced dissociation (CID) fragments by liquid chromatography electro spray ionization time-of-flight mass spectrometry (LC/ESI-TOF).
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2005.06.002