Polymorphisms of GSTM1, GSTT1, GSTP1 genes and chromosomal aberrations in lung cancer patients

• Published in: Journal of cancer research and clinical oncology 2017-11, Vol.143 (11), p.2235-2243
• Main Authors: Minina, Varvara I., Soboleva, Olga A., Glushkov, Andrey N., Voronina, Elena N., Sokolova, Ekaterina A., Bakanova, Marina L., Savchenko, Yana A., Ryzhkova, Anastasia V., Titov, Ruslan A., Druzhinin, Vladimir G., Sinitsky, Maxim Yu, Asanov, Maxim A.
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - genetics; Cancer Research; Carcinogens; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Chromosome Aberrations; Chromosomes; Data processing; Detoxification; DNA probes; Female; Follow-Up Studies; Gene frequency; Genotoxicity; Genotyping; Glutathione S-Transferase pi - genetics; Glutathione transferase; Glutathione Transferase - genetics; GSTM1 protein; GSTT1 protein; Hematology; Humans; Internal Medicine; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Lymphocytes; Male; Medicine; Medicine & Public Health; Metaphase; Middle Aged; Neoplasm Staging; Non-small cell lung carcinoma; Occupational exposure; Oncology; Original Article – Cancer Research; Pathogenesis; Peripheral blood; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic - genetics; Prognosis; Small Cell Lung Carcinoma - genetics; Small Cell Lung Carcinoma - pathology; Smoking; Survival Rate
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-017-2486-3

Purpose To study the potential links between genetic polymorphisms in the GSTT1 , GSTM1, GSTP1 genes and the frequency of chromosomal aberrations (CAs) in lung cancer patients and healthy residents in Russian Federation. Methods 200 cells in well-spread metaphase with 46 chromosomes were examined for 353 newly diagnosed lung cancer patients (males) who received medical treatment in the Kemerovo Regional Oncology Center (Kemerovo, Russian Federation), and 300 healthy males from Kemerovo, Russian Federation. The polymorphisms of the GSTM1 del and GSTT1 del genes were analysed by multiplex PCR. Genotyping of the polymorphic variants in the GSTP1 ( A313G, T341C ) gene was performed using Real-time PCR with competing TaqMan probes complementary to the polymorphic DNA sites. The data analysis was performed using software STATISTICA 8.0 (StatSoft Inc., USA). Results We discovered that a GSTM1 del polymorphism increases the frequency of chromosomal damage in smoking patients with lung cancer, a general group of lung cancer patients, donors with non-small cell lung cancer and patients in the latest stages of the malignant process. The synergetic effects of occupational exposure and the malignant process can induce some modifications in the cytogenetic status in lung cancer patients harbouring the GSTM1 del polymorphism. Conclusions CAs in peripheral blood lymphocytes can be used as biomarkers of the early biological effects of exposure to genotoxic carcinogens and may predict future cancer incidence in several epidemiologic studies. Genetic changes in genes encoding phase II detoxification enzymes are linked to decreases in the metabolic detoxification of environmentally derived genotoxic carcinogens.