Identification and functional characterization of APRR2 controlling green immature fruit color in cucumber (Cucumis sativus L.)

Our previous studies have found that gene APRR2 is the candidate gene of w controlling the green color of immature fruit in cucumber, and its allele aprr2 , which controls the white color, has a 101-amino acid residues deletion at the C-terminus compared with APRR2 . Here, we characterized the APRR2...

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Bibliographic Details
Published in:Plant growth regulation 2017-11, Vol.83 (2), p.233-243
Main Authors: Jiao, Jianqing, Liu, Hanqiang, Liu, Jia, Cui, Mingming, Xu, Jing, Meng, Huanwen, Li, Yuhong, Chen, Shuxia, Cheng, Zhihui
Format: Article
Language:English
Subjects:
Agriculture
Amino acids
APRR2 gene
Biomedical and Life Sciences
C-Terminus
Color
Cucumis sativus
Deoxyribonucleic acid
DNA
Fruits
Gene expression
Homeobox
Life Sciences
Localization
Original Paper
Phylogeny
Plant Anatomy/Development
Plant Physiology
Plant Sciences
Vegetables
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Summary:Our previous studies have found that gene APRR2 is the candidate gene of w controlling the green color of immature fruit in cucumber, and its allele aprr2 , which controls the white color, has a 101-amino acid residues deletion at the C-terminus compared with APRR2 . Here, we characterized the APRR2 and analyzed its function in cucumber. qPCR analysis and prokaryotic expression of APRR2 and aprr2 clarified the relationship between APRR2 and aprr2 and confirmed that APRR2 / aprr2 is a gene controlling cucumber color. Sequence and phylogenetic relationship analysis revealed that gene APRR2 and aprr2 with REC domain and DNA-binding B-motif are placed into subclass I of PRR subfamily. Subcellular localization assay indicated that APRR2 is a nuclear protein, but aprr2 is not. These results suggest APRR2 gene is a transcription factor in cucumber. Additionally, the expression analysis of Class-I KNOTTED1-like homeobox genes TKN4 and TKN2 involved in the chloroplast development showed they were in line with APRR2 expression, therefore, APRR2 together with TKN4, TKN2 likely contribute to the mechanisms related to chloroplast development.
ISSN:0167-6903
1573-5087
DOI:10.1007/s10725-017-0304-1