Studies on lipid A isolated from Phyllobacterium trifolii PETP02^sup T^ lipopolysaccharide

The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02T, a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, subst...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2017-11, Vol.110 (11), p.1413
Main Authors: Zamlynska, Katarzyna, Komaniecka, Iwona, Zebracki, Kamil, Mazur, Andrzej, Sroka-bartnicka, Anna, Choma, Adam
Format: Article
Language:English
Subjects:
Acids
Bacteria
Carbohydrates
Fatty acids
Genomes
Glucose
Lipid A
Lipids
Lipopolysaccharides
Nitrogen fixation
Residues
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Summary:The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02T, a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-d-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4′ of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-017-0872-0