Polypeptide Profile, Amino Acid Composition and Some Functional Properties of Calabash Nutmeg (Monodora myristica) Flour and Protein Products

The aim of this work was to compare the physicochemical and functional properties of calabash nutmeg ( Monodora myristica ) seed protein flour with those of protein-enriched products (albumin, globulin, and protein isolate). Defatted M. myristica seed flour (MMF) was used to prepare various protein...

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Bibliographic Details
Published in:Journal of the American Oil Chemists' Society 2017-11, Vol.94 (11), p.1361-1371
Main Authors: Akinyede, Adedamola I., Malomo, Sunday A., Fagbemi, Tayo N., Osundahunsi, Oluwatooyin F., Aluko, Rotimi E.
Format: Article
Language:English
Subjects:
Agriculture
Albumin
Amino acid composition
Amino acids
Biomaterials
Biotechnology
Chemistry
Chemistry and Materials Science
Dialysis
Emulsifiers
Emulsions
Flour
Foaming
Food Science
Functional properties
Gel electrophoresis
Globulin, polypeptide composition
Globulins
Industrial Chemistry/Chemical Engineering
Monodora myristica
Nutmeg
Original Paper
pH effects
Polymethyl methacrylate
Polypeptides
Properties (attributes)
Protein solubility
Proteins
Seeds
Sodium chloride
Sodium hydroxide
Solubility
Spices
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Summary:The aim of this work was to compare the physicochemical and functional properties of calabash nutmeg ( Monodora myristica ) seed protein flour with those of protein-enriched products (albumin, globulin, and protein isolate). Defatted M. myristica seed flour (MMF) was used to prepare various protein products. A NaCl extract of MMF was dialyzed against water to obtain the soluble albumin fraction (MMA) and a precipitated globulin fraction (MMG). MMF was also extracted with NaOH, the extract adjusted to pH 4.0 and the precipitated proteins collected as the isolate (MMI). Non-reducing gel electrophoresis showed that the MMF, MMG and MMI had similar composition that was dominated by 55 and 110 kDa polypeptides while MMA consisted mainly of smaller (
ISSN:0003-021X
1558-9331
DOI:10.1007/s11746-017-3043-6