Target‐Selective Fluorescence Imaging and Photocytotoxicity against H2O2 High‐Expressing Cancer Cells Using a Photoactivatable Theranostic Agent

A purpose‐designed and synthesized H2O2‐reactive and photoactivatable theranostic agent 1 consisting of 1) an arylboronic acid moiety, 2) pro‐fluorophore moiety, and 3) photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2 while simultaneously releasing resorufin f...

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Bibliographic Details
Published in:Chemistry, an Asian journal an Asian journal, 2017-10, Vol.12 (20), p.2656-2659
Main Authors: Takagi, Ryoma, Takeda, Ayano, Takahashi, Daisuke, Toshima, Kazunobu
Format: Article
Language:English
Subjects:
Chemistry
cytotoxicity
Fluorescence
fluorescent probes
Hydrogen peroxide
peroxides
sensitizers
Target recognition
theranostics
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Summary:A purpose‐designed and synthesized H2O2‐reactive and photoactivatable theranostic agent 1 consisting of 1) an arylboronic acid moiety, 2) pro‐fluorophore moiety, and 3) photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2 while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition, 1 was cell‐permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that 1 produced a selective fluorescence response to H2O2, even in living cultured cells. I'd like some H2O, too: A purpose‐designed and synthesized H2O2‐responsive and photoactivatable theranostic agent selectively and effectively reacted with H2O2, and simultaneously released resorufin for fluorescence detection. In addition, the agent was cell‐permeable, and promoted a selective fluorescence response to H2O2, even in living cultured cells. Furthermore, this agent exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation.
ISSN:1861-4728
1861-471X
DOI:10.1002/asia.201701004