Activation of IKK[alpha] target genes depends on recognition of specific [kappa]B binding sites by RelB:p52 dimers

• Published in: The EMBO journal 2004-10, Vol.23 (21), p.4202
• Main Authors: Bonizzi, Giuseppina, Bebien, Magali, Otero, Dennis C, Johnson-Vroom, Kirsten E, Cao, Yixue, Vu, Don, Jegga, Anil G, Aronow, Bruce J, Ghosh, Gourisankar, Rickert, Robert C, Karin, Michael
• Format: Article
• Language: English
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1038/sj.emboj.7600391

IkappaB Kinase (IKK)alpha is required for activation of an alternative NF-kappaB signaling pathway based on processing of the NF-kappaB2/p100 precursor protein, which associates with RelB in the cytoplasm. This pathway, which activates RelB:p52 dimers, is required for induction of several chemokine genes needed for organization of secondary lymphoid organs. We investigated the basis for the IKKalpha dependence of the induction of these genes in response to engagement of the lymphotoxin beta receptor (LTbetaR). Using chromatin immunoprecipitation, we found that the promoters of organogenic chemokine genes are recognized by RelB:p52 dimers and not by RelA:p50 dimers, the ubiquitous target for the classical NF-kappaB signaling pathway. We identified in the IKKalpha-dependent promoters a novel type of NF-kappaB-binding site that is preferentially recognized by RelB:p52 dimers. This site links induction of organogenic chemokines and other important regulatory molecules to activation of the alternative pathway.