Differential effect of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) on leukocyte infiltration during contact hypersensitivity responses

• Published in: PeerJ preprints 2017-06
• Main Authors: Early, Merideth, Schroeder, William G, Unnithan, Ranajana, Gilchrist, John M, Muller, William A, Schenkel, Alan
• Format: Article
• Language: English
• Subjects: CD31 antigen; CD4 antigen; Cell adhesion & migration; Cell adhesion molecules; Contact dermatitis; Dinitrofluorobenzene; Effector cells; Endothelial cells; Granulocytes; Hypersensitivity; Leukocyte migration; Leukocytes (granulocytic); Lymphocytes; Lymphocytes T; Natural killer cells; Platelets; Rodents
• ISSN: 2167-9843
• DOI: 10.7287/peerj.preprints.2947v2

BACKGROUND: 2’-4’ Dinitrofluorobenzene (DNFB) induced contact hypersensitivity is an established model of contact sensitivity and leukocyte migration. Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) deficient mice were used to examine the role of PECAM-1 in the migration capacity of several different leukocyte populations after primary and secondary application. RESULTS: γδ T lymphocytes, granulocytes, and Natural Killer cells were most affected by PECAM-1 deficiency at the primary site of application. γδ T lymphocytes, granulocytes, DX5+ Natural Killer cells, and, interestingly, effector CD4+ T lymphocytes were most affected by the loss of PECAM-1 at the secondary site of application. CONCLUSIONS: PECAM-1 is used by many leukocyte populations for migration, but there are clearly differential effects on the usage by each subset. Further, the overall kinetics of each population varied between primary and secondary application, with large relative increases in γδ T lymphocytes during the secondary response.