Isolating high-quality RNA from mangroves without liquid nitrogen

Mangroves form unique communities in tropical coastal regions and tidal lowlands. Isolating RNA from mangrove leaves is difficult because of high amounts of secondary metabolites and polysaccharides. Conventional extraction methods produce poor-quality mangrove RNA. We present a simple, fast, and co...

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Bibliographic Details
Published in:Plant molecular biology reporter 2004-06, Vol.22 (2), p.197-197
Main Authors: Fu, Xinhui, Deng, Shulin, Su, Guohua, Zeng, Qinglu, Shi, Suhua
Format: Article
Language:English
Subjects:
Coastal zone
Liquid nitrogen
Lowlands
Mangroves
Metabolites
Polymerase chain reaction
Polysaccharides
Production methods
Ribonucleic acid
RNA
Saccharides
Secondary metabolites
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Summary:Mangroves form unique communities in tropical coastal regions and tidal lowlands. Isolating RNA from mangrove leaves is difficult because of high amounts of secondary metabolites and polysaccharides. Conventional extraction methods produce poor-quality mangrove RNA. We present a simple, fast, and convenient protocol for isolating RNA from 5 mangrove species:Aegiceras corniculatum, Bruguiera gymnorrhiza, Ceriops tagal, Kandelia candel, andSonneratia apetala. Isolating RNA from other mangrove species is also possible. Obtained RNA was of high quality and used in an RT-PCR reaction that amplified 0.6 kb of theA. corniculatum CPI-1 gene.
ISSN:0735-9640
1572-9818
DOI:10.1007/BF02772728