dried corncob as a source of DNA for PCR analysis

We developed a procedure to isolate DNA from dried corncobs. This DNA was amplified successfully by PCR, producing well-defined bands in response to specific primers. The SSR patterns between cob and leaf DNA of the same inbred line were found to be identical, indicating that the DNA in the cob cell...

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Bibliographic Details
Published in:Plant molecular biology reporter 2002-03, Vol.20 (1), p.59-65
Main Authors: Schneerman, M.C, Mwangi, J, Hobart, B, Arbuckle, J, Vaske, D.A, Register, J.C. III, Weber, D.F
Format: Article
Language:English
Subjects:
corn
corn cobs
Deoxyribonucleic acid
DNA
DNA fingerprinting
Inbreeding
leaves
microsatellite repeats
molecular sequence data
new methods
Polymerase chain reaction
Primers
Senescence
Zea mays
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Summary:We developed a procedure to isolate DNA from dried corncobs. This DNA was amplified successfully by PCR, producing well-defined bands in response to specific primers. The SSR patterns between cob and leaf DNA of the same inbred line were found to be identical, indicating that the DNA in the cob cells had not degraded during senescence.
ISSN:0735-9640
1572-9818
DOI:10.1007/BF02801933