Intron III-Specific Markers for Screening of [beta]-amylase Alleles in Barley Cultivars

• Published in: Plant molecular biology reporter 1999-06, Vol.17 (2), p.139
• Main Author: ErkkilÄ, Maria J
• Format: Article
• Language: English
• Subjects: Alleles; Amylases; Barley; Cultivars; Enzymatic activity; Fragments; H protein; Markers; Plant breeding; Screening; β-Amylase
• ISSN: 0735-9640; 1572-9818
• DOI: 10.1023/A:1007595821379

In modern malting barley breeding it is important to increase the level of β-amylase activity level in barley. The aim of this study was to investigate if a PCR method for screening β-amy1 alleles can be used as an indicator for β-amylase activity level in barley. Activity was assayed from 24 cultivars, 7 lines, and a Hordeum spontaneum PI 296897 strain grown in the same field. The β-amy1 alleles were identified by amplifying the intron III-specific region of the gene using PCR. No new alleles were detected in addition to the three alleles found earlier: cv Adorra-like, cv Haruna Nijo-like and PI 296897-like β-amy1 allele. Samples were grouped according to the nature of their β-amy1 locus and enzyme activities were compared between the groups. Cultivars carrying a cv Haruna Nijo-like β-amy1 allele had 1.3 times and lines carrying a PI 296897-like β-amy1 allele had 2.1 times higher β-amylase activity than cultivars carrying a cv Adorra-like β-amy1 allele. The mean activities are significantly different in the allele groups (Kruskal-Wallis: for protein H= 11.54, P< 0.01; for meal H= 12.74, P< 0.01). PCR fragments can be used as allele specific markers to predict the level of β-amylase activity in breeding when such variation of the intron III is concerned.