Identification ofporphyra lines (rhodophyta) by AFLP DNA fingerprinting and molecular markers

Twenty-sevenPorphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed...

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Bibliographic Details
Published in:Plant molecular biology reporter 2005-09, Vol.23 (3), p.251-262
Main Authors: Sun, Jian Wei, De Jin, Min, Zhou, Chun Jiang, Yang, Qing Kai, Weng, Man Li, De Duan, Lin, Xu, Pu, Ma, Jia Hai, Wang, Bin
Format: Article
Language:English
Subjects:
Amplification
Amplified fragment length polymorphism
Deoxyribonucleic acid
DNA
DNA fingerprinting
DNA fingerprints
Fingerprinting
Fingerprints
Gene polymorphism
Identification
Markers
Nucleotide sequence
Plant populations
Polymorphism
Porphyra
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Summary:Twenty-sevenPorphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were scored and used to develop the DNA fingerprints of the 27Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results, computerized AFLP DNA fingerprints were constructed in which each of the 27Porphyra lines has its unique AFLP fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germ-plasm identification-AFLP) was designed for identification of the 27Porphyra lines. In addition, 21 specific AFLP markers from 15Porphyra lines were identified; 6 AFLP markers from 4Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification, and resource protection of thePorphyra lines.
ISSN:0735-9640
1572-9818
DOI:10.1007/BF02772755