In Silico and Quantitative Analyses of the Putative FLC-like Homologue in Coffee (Coffea arabica L.)

The sequential pattern of coffee flowering is a major constraint that directly affects productivity, increases harvest costs, and generates a final product of lower quality for mixing dry fruits with ripe and unripe ones. The objective of this work was to identify and analyze one of the main genes i...

Full description

Saved in:
Bibliographic Details
Published in:Plant molecular biology reporter 2012-02, Vol.30 (1), p.29-35
Main Authors: Barreto, Horllys Gomes, Lazzari, Fabiane, Ságio, Solange Aparecida, Chalfun-Junior, Antonio, Paiva, Luciano Vilela, Benedito, Vagner Augusto
Format: Article
Language:English
Subjects:
Bioinformatics
Biological evolution
Biomedical and Life Sciences
Coffea arabica
Coffee
Evolutionary conservation
Flowering
Gene expression
Gene regulation
Homology
Life Sciences
Metabolomics
Plant Breeding/Biotechnology
Plant Sciences
Proteomics
Quantitative analysis
Wildlife conservation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The sequential pattern of coffee flowering is a major constraint that directly affects productivity, increases harvest costs, and generates a final product of lower quality for mixing dry fruits with ripe and unripe ones. The objective of this work was to identify and analyze one of the main genes involved in flowering regulation, FLOWERING LOCUS C ( FLC ) in coffee ( Coffea arabica L.). The identification of this gene was conducted in silico using a coffee EST database (CAFEST) and bioinformatics tools. Quantitative PCR results suggest that the identified CaFLC-like homologue is directly involved in flowering regulation in coffee. This expands our knowledge on evolutionary conservation of flowering pathways in dicot species. The functional studies of CaFLC-like with mutants of a more tractable species will lead to a better understanding of the molecular regulation as well as the specific functions of each gene flowering during floral induction in coffee.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-011-0310-9