SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.)

Rice blast caused by the fungus Magnaporthe oryzae is one of the most devastating diseases of rice in nearly all rice growing areas of the world including Malaysia. To develop cultivars with resistance against different races of M. oryzae , availability of molecular markers along with marker-assiste...

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Bibliographic Details
Published in:Plant molecular biology reporter 2012-02, Vol.30 (1), p.79-86
Main Authors: Ashkani, Sadegh, Rafii, Mohd Yusop, Rusli, Ibrahim, Sariah, Meon, Abdullah, Siti Nor Akmar, Abdul Rahim, Harun, Latif, M. A.
Format: Article
Language:English
Subjects:
Bioinformatics
Biomedical and Life Sciences
Cultivars
Genetic crosses
Genotype & phenotype
Life Sciences
Marker-assisted selection
Markers
Metabolomics
Oryza sativa
Parents
Phenotypic variations
Plant breeding
Plant Breeding/Biotechnology
Plant Sciences
Population (statistical)
Progeny
Proteomics
Rice
Rice blast
Statistical analysis
Statistical tests
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Summary:Rice blast caused by the fungus Magnaporthe oryzae is one of the most devastating diseases of rice in nearly all rice growing areas of the world including Malaysia. To develop cultivars with resistance against different races of M. oryzae , availability of molecular markers along with marker-assisted selection strategies are essential. In this study, 11 polymorphic simple sequence repeat (SSR) markers with good fit of 1:2:1 ratio for single gene model in F 2 population derived from the cross of Pongsu seribu 2 (Resistant) and Mahsuri (Susceptible) rice cultivars were analysed in 296 F 3 families derived from individual F 2 plants to investigate association with Pi gene conferring resistance to M. oryzae pathotype. Parents and progeny were grouped into two phenotypic classes based on their blast reactions. Chi-square test for the segregation of resistance and susceptibility in F 3 generation fitted a ratio of approximately 3:1. Association of SSR markers with phenotypic trait in F 3 families was identified by statistical analysis. Four SSR markers (RM413, RM5961, RM1233 and RM8225) were significantly associated with blast resistance to pathotype 7.2 of M. oryzae in rice ( p  ≤ 0.01). These four markers accounted for about 20% of total phenotypic variation. So, these markers were confirmed as suitable markers for use in marker-assisted selection and confirmation of blast resistance genes to develop rice cultivars with durable blast resistance in Malaysian rice breeding programmes.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-011-0315-4