Effect of Ti6Al4V surface morphology on the osteogenic differentiation of human embryonic stem cells

Ti6Al4V alloys usually need to undergo some kind of surface treatment to enable good bone growth and implant integration. In this work, three treatments that modify the titanium alloy surface were studied with the aim of promoting osteogenic differentiation of human-embryonic-stem-cell-derived mesen...

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Bibliographic Details
Published in:Journal of materials research 2017-10, Vol.32 (20), p.3811-3821
Main Authors: Antonini, Leonardo Marasca, Kothe, Vinícius, Reilly, Gwendolen C., Owen, Robert, Marcuzzo, Jossano Saldanha, Malfatti, Célia de Fraga
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Applied and Technical Physics
Atomic force microscopy
Binding sites
Biocompatibility
Biomaterials
Biomedical materials
Bone marrow
Cell culture
Contact angle
Corrosion resistance
Differentiation
Electrolytes
Embryos
Inorganic Chemistry
Materials Engineering
Materials research
Materials Science
Mechanical polishing
Morphology
Nanostructured materials
Nanotechnology
Proteins
Software
Stem cells
Sulfuric acid
Surface treatment
Titanium alloys
Titanium base alloys
Topography
Transplants & implants
Wettability
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Summary:Ti6Al4V alloys usually need to undergo some kind of surface treatment to enable good bone growth and implant integration. In this work, three treatments that modify the titanium alloy surface were studied with the aim of promoting osteogenic differentiation of human-embryonic-stem-cell-derived mesenchymal progenitors (hESC-MPs). The surface treatments used were mechanical polishing and electropolishing for 4 or 12 min in an H2SO4/HF/glycerine solution. The samples were characterised by atomic force microscopy, profilometry, X-ray photoelectron spectroscopy, and wettability. Samples were seeded with hESC-MPs in osteogenic media, and the cell number and alkaline phosphatase activity were measured. The electropolishing surface treatments influenced the nanometric morphology and wettability. However, the electropolished surfaces contributed in the same way as the mechanically polished surface to osteogenic differentiation, indicating that differentiation was strongly influenced by microroughness, which did not differ among the treatments used in the present work.
ISSN:0884-2914
2044-5326
DOI:10.1557/jmr.2017.392