Microaerophilic environments improve the productivity of medium chain length polyhydroxyalkanoate biosynthesis from fatty acids in Pseudomonas putida LS46

• Published in: Process biochemistry (1991) 2017-08, Vol.59, p.18-25
• Main Authors: Blunt, Warren, Dartiailh, Christopher, Sparling, Richard, Gapes, Daniel, Levin, David B., Cicek, Nazim
• Format: Article
• Language: English
• Subjects: Aeration; Biopolymer; Biopolymers; Bioreactor; Bioreactors; Biosynthesis; Chemical synthesis; Dissolved oxygen; Fatty acids; Fed batch; Mass transfer; Microaerophilic; Octanoic acid; Oxygen; Oxygen transfer; Polyhydroxyalkanoates; Polyhydroxyalkanoic acid; Productivity; Pseudomonas putida; Studies
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2017.04.028

[Display omitted] •Mcl-PHA synthesis from fatty acids was studied in low DO environments.•Significant PHA accumulated at 1–5% DO, concurrent to growth of nearly 0.5μmax.•Further reductions in DO increased the rate of mcl-PHA synthesis.•Low DO environments are beneficial for mcl-PHA synthesis from fatty acids. Medium chain length polyhydroxyalkanoate (mcl-PHA, a commercially desirable biopolymer) synthesis from fatty acids is an oxidative process. Growth and mcl-PHA synthesis were studied in Pseudomonas putida LS46 when subjected to a spectrum of oxygen transfer rates, which were intended to simulate dissolved oxygen (DO) gradients likely to be encountered during process scale-up. It was found that maintaining DO at 1–5% (of air saturation at 30°C) initiated significant mcl-PHA synthesis and concurrent growth at