Exploring in vitro germplasm conservation options for sugarcane (Saccharum spp. hybrids) in South Africa

In vitro plantlets of sugarcane cultivar NCo310 were maintained in slow growth conditions at both 18 and 24°C and on four semi-solid media: SG1—Murashige and Skoog (MS) salts and vitamins with 20 g L−1 sucrose, SG2—½ MS with 10 g L−1 sucrose, SG3—MS with 20 g L−1 sucrose and 1 mg L−1 abscisic acid (...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2017-08, Vol.53 (4), p.402-409
Main Authors: Banasiak, M., Snyman, S. J.
Format: Article
Language:English
Subjects:
Abscisic acid
Agricultural biotechnology
Aluminum
Biomedical and Life Sciences
Cell Biology
CRYOPRESERVATION
Cultivars
Developmental Biology
Germplasm
Growth conditions
Hybrids
Life Sciences
Meristems
Methods
Multiplication
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Plantlets
Saccharum
Salts
Semisolids
Shoots
Subculture
Sucrose
Sugar
Sugarcane
Survival
Temperature
Vitamins
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Summary:In vitro plantlets of sugarcane cultivar NCo310 were maintained in slow growth conditions at both 18 and 24°C and on four semi-solid media: SG1—Murashige and Skoog (MS) salts and vitamins with 20 g L−1 sucrose, SG2—½ MS with 10 g L−1 sucrose, SG3—MS with 20 g L−1 sucrose and 1 mg L−1 abscisic acid (ABA), and SG4—½ MS with 10 g L−1 sucrose and 1 mg L−1 ABA. After 8, 12, 24, 36, and 48 mo shoot multiplication rates were recorded, shoots were removed from storage and subcultured every 2 wk on SG1 with 0.015 mg L−1 kinetin and 0.1 mg L−1 benzyl aminopurine for 2 mo. At 18°C, all media supported storage for 48 mo with subculturing every 12 mo. Shoot multiplication post-retrieval was significantly higher on the SG2 medium compared with the non-stored control (362 ± 84 and 126 ± 26 shoots per recovered shoot after 2 mo, respectively). In addition, shoots could be maintained for 48 mo on SG2 medium with one subculture without compromising post-storage multiplication ability. At 24°C, storage on all four media supported recovery and multiplication of shoots for 8 mo and only SG2 medium facilitated survival for 12 mo. There was no advantage to incorporating ABA into the storage media, regardless of the temperature and storage time. Cryopreservation of cultivar NCo376 in vitro-derived shoot meristems using the V-cryo-plate method demonstrated that the sucrose concentration in the loading solution (0.8–1.8 M) had no significant effect on survival of the meristems, which ranged from 41.7 ± 4.8 to 69.4 ± 10%.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-017-9853-2