Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study

OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a high...

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Bibliographic Details
Published in:Revista odonto ciência 2017, Vol.32 (1), p.28
Main Authors: Wanderley, Flávia Godinho, Nunes, Carlla Silva, Ferreira, Maira Sá, Gonzalez, Ana Cristina, Reis, Silvia Regina Almeida, Medrado, Alena Ribeiro Alves Peixoto
Format: Article
Language:English
Subjects:
Actin
Angiogenesis
Antibodies
Antigens
Blood vessels
Cancer
Capillaries
Comparative analysis
Dysplasia
Education
Extracellular matrix
Histomorphometry
Laboratories
Metastasis
Monoclonal antibodies
Mucosa
Neuronal-glial interactions
Pathology
Pericytes
Proteins
Radiation
Smooth muscle
Squamous cell carcinoma
Statistical analysis
Tumors
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Summary:OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a higher education institution. Tissue sections from patients diagnosed with actinic cheilitis and carcinoma were immunohistochemically analyzed using monoclonal antibodies specific for smooth muscle alpha actin (SMA) and neuron-glial antigen 2 (NG2). Individual cells that stained positive for these proteins were counted in specific fields by means of histomorphometry.RESULTS: Subsequent statistical analysis revealed a higher number of NG2-positive when compared with SMA-positive cells in both lesion types (p = 0.005) and these cells were located adjacent to blood vessels. The differences between normal oral mucosa, severe dysplasia, and carcinoma specimens were statistically significant (p = 0.009; p = 0.006).CONCLUSION: These data strongly suggest that increased expression of NG2- and SMA-positive cells is associated with formation of blood capillaries, highlighting the importance of angiogenesis in tumor progression.
ISSN:0102-9460
1980-6523
DOI:10.15448/1980-6523.2017.1.23344