Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study

OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a high...

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Published in:Revista odonto ciência 2017, Vol.32 (1), p.28
Main Authors: Wanderley, Flávia Godinho, Nunes, Carlla Silva, Ferreira, Maira Sá, Gonzalez, Ana Cristina, Reis, Silvia Regina Almeida, Medrado, Alena Ribeiro Alves Peixoto
Format: Article
Language:English
Subjects:
Actin
Angiogenesis
Antibodies
Antigens
Blood vessels
Cancer
Capillaries
Comparative analysis
Dysplasia
Education
Extracellular matrix
Histomorphometry
Laboratories
Metastasis
Monoclonal antibodies
Mucosa
Neuronal-glial interactions
Pathology
Pericytes
Proteins
Radiation
Smooth muscle
Squamous cell carcinoma
Statistical analysis
Tumors
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container_start_page 28
container_title Revista odonto ciência
container_volume 32
creator Wanderley, Flávia Godinho
Nunes, Carlla Silva
Ferreira, Maira Sá
Gonzalez, Ana Cristina
Reis, Silvia Regina Almeida
Medrado, Alena Ribeiro Alves Peixoto
description OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a higher education institution. Tissue sections from patients diagnosed with actinic cheilitis and carcinoma were immunohistochemically analyzed using monoclonal antibodies specific for smooth muscle alpha actin (SMA) and neuron-glial antigen 2 (NG2). Individual cells that stained positive for these proteins were counted in specific fields by means of histomorphometry.RESULTS: Subsequent statistical analysis revealed a higher number of NG2-positive when compared with SMA-positive cells in both lesion types (p = 0.005) and these cells were located adjacent to blood vessels. The differences between normal oral mucosa, severe dysplasia, and carcinoma specimens were statistically significant (p = 0.009; p = 0.006).CONCLUSION: These data strongly suggest that increased expression of NG2- and SMA-positive cells is associated with formation of blood capillaries, highlighting the importance of angiogenesis in tumor progression.
doi_str_mv 10.15448/1980-6523.2017.1.23344
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ispartof Revista odonto ciência, 2017, Vol.32 (1), p.28
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1980-6523
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subjects Actin
Angiogenesis
Antibodies
Antigens
Blood vessels
Cancer
Capillaries
Comparative analysis
Dysplasia
Education
Extracellular matrix
Histomorphometry
Laboratories
Metastasis
Monoclonal antibodies
Mucosa
Neuronal-glial interactions
Pathology
Pericytes
Proteins
Radiation
Smooth muscle
Squamous cell carcinoma
Statistical analysis
Tumors
title Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study
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