Lactobacillus Plantarum CGMCC8198-Fermented Milk Inhibits Melanogenesis in B16F10 Melanoma Cells

Abnormal accumulation of melanin causes unaesthetic hyperpigmentation. Many studies suggest that yogurt have some effects of antioxidant and anti-aging. In this study, the antioxidant and melanogenesis-inhibitory activity of fermented milk supernatant (FMS) prepared by Lactobacillus plantarum CGMCC8...

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Bibliographic Details
Published in:Food Science and Technology Research 2017, Vol.23(5), pp.669-678
Main Authors: Zhang, Cai-Jiao, Pan, Li-Juan, Lu, Wen-Han, Zhou, Hao, Wang, Nan, Zhang, Tong-Cun, Luo, Xue-Gang
Format: Article
Language:English
Subjects:
Aging
antioxidant
Antioxidants
Fermented milk products
Flexible manufacturing systems
Hyperpigmentation
Immunocytochemistry
Lactobacillus plantarum
lactobacillus-fermented milk
Melanin
melanogenesis
Melanoma
Microphthalmia-associated transcription factor
Milk
MITF
Polymerase chain reaction
Probiotics
Transcription
Tyrosinase
Yogurt
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Summary:Abnormal accumulation of melanin causes unaesthetic hyperpigmentation. Many studies suggest that yogurt have some effects of antioxidant and anti-aging. In this study, the antioxidant and melanogenesis-inhibitory activity of fermented milk supernatant (FMS) prepared by Lactobacillus plantarum CGMCC8198, a novel probiotics strain, were detected. The results in both ABTS and DPPH assay showed that FMS exhibited markedly antioxidant effect. Melanin production was significantly reduced by FMS, while the survival of cells was not affected. Furthermore, FMS reduced the activity of cellular tyrosinase but had no effect on tyrosinase itself, and the results of RT-PCR, Western blot and immunocytochemistry demonstrated that FMS could inhibit the transcription and expression of tyrosinase, TRP-1, TRP-2 and MITF, and these effects were mainly depended on heated-resistant macromolecule substances. Our results suggested that Lactobacillus plantarum CGMCC8198-Fermented Milk could be a good candidate for antioxidant and treating hyperpigmentation disorders and could also be used as a cosmetic whitening agent.
ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.23.669