Protoplasts in the analysis of early plant-pathogen interactions: current applications and perspectives

Transient gene expression in protoplasts has been applied to the analysis of gene expression, promoter activity, subcellular localization of proteins, and genome editing. Its advantages have been realized in recent years in various physiological, biochemical, genetic, and molecular biological studie...

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Bibliographic Details
Published in:European journal of plant pathology 2017-12, Vol.149 (4), p.1001-1010
Main Authors: Xing, Tim, Li, Xiu-Qing, Laroche, Andre, Tian, Lining, Tubei, Kipkios, Wang, Xiaojing
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Ecology
Functional analysis
Gene expression
Genomes
Life Sciences
Localization
Pathogens
Plant Pathology
Plant Sciences
Proteins
Protoplasts
Signaling
Urban regeneration
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Summary:Transient gene expression in protoplasts has been applied to the analysis of gene expression, promoter activity, subcellular localization of proteins, and genome editing. Its advantages have been realized in recent years in various physiological, biochemical, genetic, and molecular biological studies. The omics and systems biology studies of plant development and their interactions with the environment have revitalized this approach. Early plant-pathogen interaction represents one of the most unique signalling research areas. Here, we try to discuss some developments and current applications of the protoplast system. It is for sure that the system will significantly bridge the gap between high throughput approaches and functional analysis in the study of plant-pathogen interactions in the omics era.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-017-1230-9