Three-dimensional nanofiber scaffolds with arrayed holes for engineering skin tissue constructs

Three-dimensional (3D) scaffolds composed of poly(ε-caprolactone) and gelatin nanofibers were fabricated by a combination of electrospinning and modified gas-foaming. Arrayed holes throughout the scaffold were created using a punch under cryo conditions. The crosslinking with glutaraldehyde vapor im...

Full description

Saved in:
Bibliographic Details
Published in:MRS communications 2017-09, Vol.7 (3), p.361-366
Main Authors: Fu, Lina, Xie, Jingwei, Carlson, Mark A., Reilly, Debra A.
Format: Article
Language:English
Subjects:
Biomaterials
Biomaterials for 3D Cell Biology Research Letter
Biomaterials for 3D Cell Biology Research Letters
Biomedical materials
Characterization and Evaluation of Materials
Construction engineering
Crosslinking
Fibroblasts
Fluorescence
Foaming
Glutaraldehyde
Materials Engineering
Materials Science
Membranes
Morphology
Nanofibers
Nanotechnology
Plastic surgery
Polymer Sciences
SCAFFOLDS
Scanning electron microscopy
Skin
Skin & tissue grafts
Spheroids
Stem cells
Tissue engineering
Water stability
Wound healing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Three-dimensional (3D) scaffolds composed of poly(ε-caprolactone) and gelatin nanofibers were fabricated by a combination of electrospinning and modified gas-foaming. Arrayed holes throughout the scaffold were created using a punch under cryo conditions. The crosslinking with glutaraldehyde vapor improved the water stability of the scaffolds. Cell spheroids of green fluorescent protein-labeled human dermal fibroblasts were prepared and seeded into the holes. It was found that the fibroblasts adhered well on the surface of nanofibers and migrated into the scaffolds due to the porous structures. The 3D nanofiber scaffolds may hold great potential for engineering tissue constructs for various applications.
ISSN:2159-6859
2159-6867
DOI:10.1557/mrc.2017.49