Hemagglutinating and acid phosphatase (AcPASE) activities in developing seedlings of four species of Cucurbitaceae

The acid phosphatase and hemagglutinating activities of four species of Cucurbitaceae were determined during seeds germination and seedlings development. In all cases traces of enzyme and hemagglutinating activities were found in dry and imbibided seeds. In developing seedlings of Cucumis sativus th...

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Bibliographic Details
Published in:Acta Societatis Botanicorum Poloniae 1999-01, Vol.68 (4), p.291-297
Main Authors: Lorenc-Kubis, Irena, Uram-Walaszczyk, Urszula
Format: Article
Language:English
Subjects:
acid phophatases
Acid phosphatase
Antibodies
Cotyledons
Cucumis sativus
Cucurbita pepo
Cucurbitaceae
Germination
immunological-relationship
Lectins
Phosphatase
Proteins
seedling development
Seedlings
Seeds
Species
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Summary:The acid phosphatase and hemagglutinating activities of four species of Cucurbitaceae were determined during seeds germination and seedlings development. In all cases traces of enzyme and hemagglutinating activities were found in dry and imbibided seeds. In developing seedlings of Cucumis sativus the activities increased to maximum on the 3rd day while in other species on the 6th day of germination and than fell down. Dot blot and Western blot techniques have shown that in seeds and seedlings of all investigated species present were proteins which cross-reacted with antibodies raised against lectins: CLBa and Con A. It has been shown that proteins from seeds and seedlings of Cucurbita maxima var. bambino, Cucurbita pepo var. giromontia and Cucumis sativus had more pronounced antigenical similarity to lectin CLBa (from Cucurbitaceae) than Con A, while proteins from cotyledons of Cucurbita pepo var. patissonina reacted better with antibodies raised against Con A (the lectin from Papilionaceae) than with CLBa.
ISSN:0001-6977
2083-9480
DOI:10.5586/asbp.1999.041