Accumulation of ixerin F and activities of some terpenoid bisynthetic enzymes in a cell suspension culture of Lactuca virosa L

• Published in: Acta Societatis Botanicorum Poloniae 2014-01, Vol.66 (2), p.185-188
• Main Authors: Stojakowska, Anna, Malarz, Janusz, van der Heijden, Robert, Verpoorte, Robert
• Format: Article
• Language: English
• Subjects: 3S-hydroxy-3-methylglutaryl-coenzyme A synthase (E.C. 4.1.3.5); acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9); Cell culture; Coenzyme A; High-performance liquid chromatography; ixerin F; Lactones; Lactuca virosa; Lactuca virosa L; Sesquiterpene lactones; Stationary phase; Suspension culture; Thiolase
• ISSN: 0001-6977; 2083-9480
• DOI: 10.5586/asbp.1997.023

A cell suspension culture of Lactuca virosa L. (Asteraceae, tribe Lactuceae) is capable of synthesizing sesquiterpene lactones of which ixerin F is the main compound. The culture was characterized on growth (by dissimilation rates), on ixerin F accumulation (by RP-HPLC) and on some enzyme activities involved in early steps of terpenoid biosynthesis. Acetoacetyl-coenzyme A thiolase (AACT, E.C. 2.3.1.9) and 3S-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS, E.C. 4.13.5) activities of the cells were assayed spectrophotometrically. HMGS activity increased during the culture period and reached a maximum during the stationary phase (190 pkat/mg protein), while AACT showed relatively high level of activity throughout the growth cycle, with transient decrease at the logarithmic growth phase and the beginning of stationary phase. Ixerin F accumulated inside the cells and the maximum concentration of 0.08% (on dry weight basis) was found in the early stationary phase of the growth cycle of the culture.