Hairy root culture of Lactuca virosa L

A hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of di...

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Bibliographic Details
Published in:Acta Societatis Botanicorum Poloniae 2014-01, Vol.64 (1), p.33-39
Main Authors: Stojakowska, Anna, Malarz, Janusz, Kisiel, Wanda, Kohlmünzer, Stanisław
Format: Article
Language:English
Subjects:
Agrobacterium rhizogenes
Biomass
Explants
Growth rate
Hairy root
hairy roots
Lactones
Lactuca virosa
Lactuca virosa L
Sesquiterpene lactones
Sucrose
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Summary:A hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant.
ISSN:0001-6977
2083-9480
DOI:10.5586/asbp.1995.006