Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]

Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompet...

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Bibliographic Details
Published in:Acta Societatis Botanicorum Poloniae 1983-01, Vol.52 (3/4), p.253-263
Main Authors: Freudenrich, C.C, Dashek, W.V
Format: Article
Language:English
Subjects:
Acids
aleurone layers
Gibberellic acid
Glutamic acid
Hordeum vulgare
Hydroxyproline
plant biochemistry
plant physiology
Proline
α-Amylase
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Summary:Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompetitively inhibited alpha-amylase. Thus, the inhibition is probably an assay artifact resulting from the formation of an enzyme-substrate-hydroxyproline or -proline complex.
ISSN:0001-6977
2083-9480
DOI:10.5586/asbp.1983.028