Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]

Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompet...

Full description

Saved in:
Bibliographic Details
Published in:Acta Societatis Botanicorum Poloniae 1983-01, Vol.52 (3/4), p.253-263
Main Authors: Freudenrich, C.C, Dashek, W.V
Format: Article
Language:English
Subjects:
Acids
aleurone layers
Gibberellic acid
Glutamic acid
Hordeum vulgare
Hydroxyproline
plant biochemistry
plant physiology
Proline
α-Amylase
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c308t-fc32ebe71b1eebf3047cd580b721f9ad1c247c2cca1a42b1c09962cce3ba1d563
cites
container_end_page 263
container_issue 3/4
container_start_page 253
container_title Acta Societatis Botanicorum Poloniae
container_volume 52
creator Freudenrich, C.C
Dashek, W.V
description Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompetitively inhibited alpha-amylase. Thus, the inhibition is probably an assay artifact resulting from the formation of an enzyme-substrate-hydroxyproline or -proline complex.
doi_str_mv 10.5586/asbp.1983.028
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_proquest_journals_1962372602</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_ff64624029c1498e86b5f92d3f2e334e</doaj_id><sourcerecordid>1962372602</sourcerecordid><originalsourceid>FETCH-LOGICAL-c308t-fc32ebe71b1eebf3047cd580b721f9ad1c247c2cca1a42b1c09962cce3ba1d563</originalsourceid><addsrcrecordid>eNpdUU1r3DAQNaWBLkmOubaCnr0ZfdiWjiW03cBCCm1OIYiRNdp48a62kh3qf1_RbS89zbzHmzfDvKq64bBuGt3eYnanNTdarkHoN9VKgJa1URreVisA4HVruu5ddZ3zvkBoAEzTraq0WXyKv5ZTiuNwJIZHz_71w_FlcMPEcDy9YI2HZcRMLKR4YEOOI07kmcM00sKeNjF5mg_sdR53mIht189ljuYUi9GIC6XMnjKRz89X1UXAMdP133pZPX75_ONuU28fvt7ffdrWvQQ91aGXghx13HEiFySorveNBtcJHgx63ovCiL5Hjko43oMxbYEkHXLftPKyuj_7-oh7e0rDAdNiIw72DxHTzmKahn4kG0KrWqFAmJ4ro0m3rglGeBkESamoeH08e5Xf_JwpT3Yf53Qs51tetspOtCCK6sNZFTBa3KUh28fvArgEwaWSHIri_f-Kb9uSmipxaK07-RtC44pO</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1962372602</pqid></control><display><type>article</type><title>Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><creator>Freudenrich, C.C ; Dashek, W.V</creator><creatorcontrib>Freudenrich, C.C ; Dashek, W.V ; SEAMEO Regional Center for Tropical Biology, Bogor (Indonesia) ; West Virginia Univ., Morgantown (USA). Dept. of Biology</creatorcontrib><description>Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompetitively inhibited alpha-amylase. Thus, the inhibition is probably an assay artifact resulting from the formation of an enzyme-substrate-hydroxyproline or -proline complex.</description><identifier>ISSN: 0001-6977</identifier><identifier>EISSN: 2083-9480</identifier><identifier>DOI: 10.5586/asbp.1983.028</identifier><language>eng</language><publisher>Warsaw: Polish Botanical Society</publisher><subject>Acids ; aleurone layers ; Gibberellic acid ; Glutamic acid ; Hordeum vulgare ; Hydroxyproline ; plant biochemistry ; plant physiology ; Proline ; α-Amylase</subject><ispartof>Acta Societatis Botanicorum Poloniae, 1983-01, Vol.52 (3/4), p.253-263</ispartof><rights>Copyright (c) 2014 Craig C. Freudenrich, William V. Dashek. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0 (the “License”). Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c308t-fc32ebe71b1eebf3047cd580b721f9ad1c247c2cca1a42b1c09962cce3ba1d563</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1962372602?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590</link.rule.ids></links><search><creatorcontrib>Freudenrich, C.C</creatorcontrib><creatorcontrib>Dashek, W.V</creatorcontrib><creatorcontrib>SEAMEO Regional Center for Tropical Biology, Bogor (Indonesia)</creatorcontrib><creatorcontrib>West Virginia Univ., Morgantown (USA). Dept. of Biology</creatorcontrib><title>Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]</title><title>Acta Societatis Botanicorum Poloniae</title><description>Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompetitively inhibited alpha-amylase. Thus, the inhibition is probably an assay artifact resulting from the formation of an enzyme-substrate-hydroxyproline or -proline complex.</description><subject>Acids</subject><subject>aleurone layers</subject><subject>Gibberellic acid</subject><subject>Glutamic acid</subject><subject>Hordeum vulgare</subject><subject>Hydroxyproline</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>Proline</subject><subject>α-Amylase</subject><issn>0001-6977</issn><issn>2083-9480</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdUU1r3DAQNaWBLkmOubaCnr0ZfdiWjiW03cBCCm1OIYiRNdp48a62kh3qf1_RbS89zbzHmzfDvKq64bBuGt3eYnanNTdarkHoN9VKgJa1URreVisA4HVruu5ddZ3zvkBoAEzTraq0WXyKv5ZTiuNwJIZHz_71w_FlcMPEcDy9YI2HZcRMLKR4YEOOI07kmcM00sKeNjF5mg_sdR53mIht189ljuYUi9GIC6XMnjKRz89X1UXAMdP133pZPX75_ONuU28fvt7ffdrWvQQ91aGXghx13HEiFySorveNBtcJHgx63ovCiL5Hjko43oMxbYEkHXLftPKyuj_7-oh7e0rDAdNiIw72DxHTzmKahn4kG0KrWqFAmJ4ro0m3rglGeBkESamoeH08e5Xf_JwpT3Yf53Qs51tetspOtCCK6sNZFTBa3KUh28fvArgEwaWSHIri_f-Kb9uSmipxaK07-RtC44pO</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Freudenrich, C.C</creator><creator>Dashek, W.V</creator><general>Polish Botanical Society</general><scope>FBQ</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>19830101</creationdate><title>Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]</title><author>Freudenrich, C.C ; Dashek, W.V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-fc32ebe71b1eebf3047cd580b721f9ad1c247c2cca1a42b1c09962cce3ba1d563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Acids</topic><topic>aleurone layers</topic><topic>Gibberellic acid</topic><topic>Glutamic acid</topic><topic>Hordeum vulgare</topic><topic>Hydroxyproline</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>Proline</topic><topic>α-Amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freudenrich, C.C</creatorcontrib><creatorcontrib>Dashek, W.V</creatorcontrib><creatorcontrib>SEAMEO Regional Center for Tropical Biology, Bogor (Indonesia)</creatorcontrib><creatorcontrib>West Virginia Univ., Morgantown (USA). Dept. of Biology</creatorcontrib><collection>AGRIS</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Acta Societatis Botanicorum Poloniae</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freudenrich, C.C</au><au>Dashek, W.V</au><aucorp>SEAMEO Regional Center for Tropical Biology, Bogor (Indonesia)</aucorp><aucorp>West Virginia Univ., Morgantown (USA). Dept. of Biology</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]</atitle><jtitle>Acta Societatis Botanicorum Poloniae</jtitle><date>1983-01-01</date><risdate>1983</risdate><volume>52</volume><issue>3/4</issue><spage>253</spage><epage>263</epage><pages>253-263</pages><issn>0001-6977</issn><eissn>2083-9480</eissn><abstract>Both 1 mM hydroxyproline and proline inhibited extracellular alpha-amylase activity without affecting its intracellular activity. This suggested that neither hydroxyproline nor proline impaired the release of alpha-amylase. Lineweaver-Burk plots revealed that both hydroxyproline and proline uncompetitively inhibited alpha-amylase. Thus, the inhibition is probably an assay artifact resulting from the formation of an enzyme-substrate-hydroxyproline or -proline complex.</abstract><cop>Warsaw</cop><pub>Polish Botanical Society</pub><doi>10.5586/asbp.1983.028</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0001-6977
ispartof Acta Societatis Botanicorum Poloniae, 1983-01, Vol.52 (3/4), p.253-263
issn 0001-6977
2083-9480
language eng
recordid cdi_proquest_journals_1962372602
source Publicly Available Content Database (Proquest) (PQ_SDU_P3)
subjects Acids
aleurone layers
Gibberellic acid
Glutamic acid
Hordeum vulgare
Hydroxyproline
plant biochemistry
plant physiology
Proline
α-Amylase
title Hydroxyproline and proline inhibit alpha-amylase from isolated barley [Hordeum vulgare L.] aleurone layers [seeds]
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T06%3A36%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hydroxyproline%20and%20proline%20inhibit%20alpha-amylase%20from%20isolated%20barley%20%5BHordeum%20vulgare%20L.%5D%20aleurone%20layers%20%5Bseeds%5D&rft.jtitle=Acta%20Societatis%20Botanicorum%20Poloniae&rft.au=Freudenrich,%20C.C&rft.aucorp=SEAMEO%20Regional%20Center%20for%20Tropical%20Biology,%20Bogor%20(Indonesia)&rft.date=1983-01-01&rft.volume=52&rft.issue=3/4&rft.spage=253&rft.epage=263&rft.pages=253-263&rft.issn=0001-6977&rft.eissn=2083-9480&rft_id=info:doi/10.5586/asbp.1983.028&rft_dat=%3Cproquest_doaj_%3E1962372602%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c308t-fc32ebe71b1eebf3047cd580b721f9ad1c247c2cca1a42b1c09962cce3ba1d563%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1962372602&rft_id=info:pmid/&rfr_iscdi=true