Preliminary studies on ribonucleases from Poa pratensis seeds

Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1. The enzymes were stable at 50°C, at pH 7.1. The activity of ribonucleases I, II...

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Bibliographic Details
Published in:Acta Societatis Botanicorum Poloniae 2015-01, Vol.43 (4), p.471-478
Main Authors: Lorenc-Kubis, Irena, Morawiecka, Bronisława
Format: Article
Language:English
Subjects:
Acetic acid
Calcium
Cellulose
Enzymes
Magnesium
pH effects
Poa pratensis
Ribonuclease IV
Seeds
Turfgrasses
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Summary:Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1. The enzymes were stable at 50°C, at pH 7.1. The activity of ribonucleases I, II, III and V were optimal at pH 7.1-7.3, and that of ribonuclease IV at pH 8.1. Ali enzymes were inhibited by Ca2+ and EDTA. Mg2+ inhibited the activity of ribonucleases II, III, IV, and had no influence on that of ribonucleases I and V. Ribonucleases IV and V showed only one activity band in disc electrophoresis, whereas ribonucleases, I, II and III were found to be heterogenous.
ISSN:0001-6977
2083-9480
DOI:10.5586/asbp.1974.044