Proteomic and bioinformatic analyses of putative Mannheimia haemolytica secretome by liquid chromatography and tandem mass spectrometry

• Published in: Veterinary microbiology 2017-05, Vol.203, p.73-80
• Main Authors: Ayalew, Sahlu, Confer, Anthony W., Hartson, Steve D., Canaan, Patricia J., Payton, Mark, Couger, Brian
• Format: Article
• Language: English
• Subjects: Animal vaccines; Animals; Bacteria; bioinformatics; bovine respiratory disease; Bovine Respiratory Disease Complex - diagnosis; Bovine Respiratory Disease Complex - microbiology; Cattle; Chromatography, Liquid - veterinary; Classical pathway; Computational Biology; Immune response; LC–MS/MS; LipoP; Liquid chromatography; Livestock; livestock and meat industry; Livestock industry; Mannheimia haemolytica; Mannheimia haemolytica - isolation & purification; Mass spectrometry; Mass spectroscopy; Membrane proteins; outer membrane proteins; Pasteurellaceae Infections - diagnosis; Pasteurellaceae Infections - microbiology; Pasteurellaceae Infections - veterinary; Phobius SP and PRED-TAT; protein secretion; Proteins; Proteomics; PSORTb; Respiratory diseases; Secretome; SecretomeP; Secretory peptides; signal peptide; SignalP 4.1; Strains (organisms); tandem mass spectrometry; Tandem Mass Spectrometry - veterinary; TatP; United States; Vaccines
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2017.02.011

•M. haemolytica secretome was characterized by liquid chromatography and tandem mass spectrometry.•A total of 923 proteins were identified in the secretome.•Screening with bioinformatic tools revealed 238 proteins are identified as putative secreted proteins.•These proteins included several proteins that have been characterized as immunogens in M. haemolytica and other bacterial species.•Potential use of secretome in design and development of potential multivalent vaccine against infection by M. haemolytica is discussed. Mannheimia haemolytica is a major bacterial contributor to bovine respiratory disease complex that costs the livestock industry a billion dollars a year in USA. Commercial vaccines are only partially efficacious under field conditions. Earlier studies found that outer membrane protein preparations and culture supernatants can induce immune responses that enhance resistance to challenge by M. haemolytica strains. The objective of this study was to characterize secretome of two M. haemolytica stains grown under two different media. Bacteria-free concentrated supernatants from M. haemolytica culture was subjected to LC–MS/MS. The secretome of M. haemolytica from both strains yielded 923 proteins. Using bioinformatic tools, 283 were identified as secreted proteins. Further breakdown of 283 proteins showed that 114 (40.2%), 184 (65.0%), 138(48.7%), 151 (53.3%) and 172 (60.7%) were characterized as secreted proteins by SignalP 4.1, SecretomeP 2.0, LipoP, Phobius, and PRED-TAT, respectively. A total of 95 (33.56%) proteins were characterized as being secreted via non-classical pathway as opposed to the majority that were secreted in signal peptide dependent pathway. The demonstrated proteins include all previously immunologically characterized M. haemolytica proteins. The potential of using secretome analysis in the design and development of a multivalent vaccine is discussed.