Health survey and assessment of the polymorphisms BRCA1/P871L, BRCA1/Q356R, and BRCA2/N372H in female gas station workers in Rio de Janeiro

Gas station workers are exposed to chemicals known to be carcinogenic, especially benzene. The objective was to analyze the health problems of female gas station workers by means of sociodemographic and clinical questionnaires, and laboratorial exams. We performed the genotyping of the polymorphisms...

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Bibliographic Details
Published in:Environmental and molecular mutagenesis 2017-12, Vol.58 (9), p.730-734
Main Authors: Silvestre, Rafaele T., Delmonico, Lucas, Bravo, Maryah, Santiago, Fábio, Scherrer, Luciano R., Moreira, Aline dos Santos, Tabalipa, Marianne, Otero, Ubirani, Ornellas, Maria Helena F., Alves, Gilda, Snyder, R.
Format: Article
Language:English
Subjects:
Adult
Alcohol Drinking - adverse effects
Alcoholic beverages
Alkanes - toxicity
Benzene
benzene toxicity
blood
Brazil
BRCA1 protein
BRCA1 Protein - genetics
BRCA2 protein
BRCA2 Protein - genetics
Breast cancer
Breast Neoplasms - chemically induced
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Carcinogens
Female
gas station
Genetic Predisposition to Disease
Genotype
Genotyping
Health problems
Humans
Miscarriage
Monocytes
Occupational Exposure
Occupational health
Polymerase chain reaction
Polymorphism
Polymorphism, Single Nucleotide
Restriction fragment length polymorphism
Service stations
Smoking
Statistical analysis
Tobacco
Tobacco Use - adverse effects
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Summary:Gas station workers are exposed to chemicals known to be carcinogenic, especially benzene. The objective was to analyze the health problems of female gas station workers by means of sociodemographic and clinical questionnaires, and laboratorial exams. We performed the genotyping of the polymorphisms BRCA1/P871L and BRCA1/Q356R by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism, and of variant allele BRCA2/N372H through direct sequencing. The female workers showed a higher concentration of monocytes (P = 0.039); a greater number of spontaneous abortions (P = 0.025, OR = 4.977, 95% CI = 1.135–30.669); higher tobacco consumption (P = 0.013); and higher alcohol consumption (P = 0.05). The statistical analysis of the polymorphisms associated with the variables monocyte concentration and miscarriage number did not reveal a significant relationship, and smoking and spontaneous abortion were not statistically associated either. Environ. Mol. Mutagen. 58:730–734, 2017. © 2017 Wiley Periodicals, Inc.
ISSN:0893-6692
1098-2280
DOI:10.1002/em.22120