Health survey and assessment of the polymorphisms BRCA1/P871L, BRCA1/Q356R, and BRCA2/N372H in female gas station workers in Rio de Janeiro

• Published in: Environmental and molecular mutagenesis 2017-12, Vol.58 (9), p.730-734
• Main Authors: Silvestre, Rafaele T., Delmonico, Lucas, Bravo, Maryah, Santiago, Fábio, Scherrer, Luciano R., Moreira, Aline dos Santos, Tabalipa, Marianne, Otero, Ubirani, Ornellas, Maria Helena F., Alves, Gilda, Snyder, R.
• Format: Article
• Language: English
• Subjects: Adult; Alcohol Drinking - adverse effects; Alcoholic beverages; Alkanes - toxicity; Benzene; benzene toxicity; blood; Brazil; BRCA1 protein; BRCA1 Protein - genetics; BRCA2 protein; BRCA2 Protein - genetics; Breast cancer; Breast Neoplasms - chemically induced; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Carcinogens; Female; gas station; Genetic Predisposition to Disease; Genotype; Genotyping; Health problems; Humans; Miscarriage; Monocytes; Occupational Exposure; Occupational health; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide; Restriction fragment length polymorphism; Service stations; Smoking; Statistical analysis; Tobacco; Tobacco Use - adverse effects
• ISSN: 0893-6692; 1098-2280
• DOI: 10.1002/em.22120

Gas station workers are exposed to chemicals known to be carcinogenic, especially benzene. The objective was to analyze the health problems of female gas station workers by means of sociodemographic and clinical questionnaires, and laboratorial exams. We performed the genotyping of the polymorphisms BRCA1/P871L and BRCA1/Q356R by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism, and of variant allele BRCA2/N372H through direct sequencing. The female workers showed a higher concentration of monocytes (P = 0.039); a greater number of spontaneous abortions (P = 0.025, OR = 4.977, 95% CI = 1.135–30.669); higher tobacco consumption (P = 0.013); and higher alcohol consumption (P = 0.05). The statistical analysis of the polymorphisms associated with the variables monocyte concentration and miscarriage number did not reveal a significant relationship, and smoking and spontaneous abortion were not statistically associated either. Environ. Mol. Mutagen. 58:730–734, 2017. © 2017 Wiley Periodicals, Inc.