Blockade of Sensorimotor Impairments in Mice with Striate Dopaminergic Deficiency by Extracts of the Darkling Beetle Alphitobius Diaperinus

• Published in: Neuroscience and behavioral physiology 2017-07, Vol.47 (6), p.690-693
• Main Authors: Koval’zon, V. M., Ushakova, N. A., Bastrakov, A. I., Kozlova, A. A., Ambaryan, A. V., Shevchenko, V. P., Nagaev, I. Yu, Pavlov, D. S.
• Format: Article
• Language: English
• Subjects: Animals; Behavioral Sciences; Biomass; Biomedical and Life Sciences; Biomedicine; Brain diseases; Dopamine receptors; Feed additives; Feeds; Mice; MPTP; Neurobiology; Neurosciences; Parkinson's disease; Plant extracts; Sensorimotor system
• ISSN: 0097-0549; 1573-899X
• DOI: 10.1007/s11055-017-0456-5

Five groups of C57Bl/6JSto mice (groups 1–5) were tested on a vertical pole two weeks after s.c. administration (groups 2–5) of the proneurotoxin methylphenyltetrahydropyridine (MPTP) at a dose of 40 mg per kg, which at this time induces sensorimotor impairments similar to the early stage of Parkinson’s disease. Mice of group 1 served as controls. One week before MPTP administration and throughout the whole of the post-treatment period (two weeks), animals of groups 3–5 received a feed supplement (8 g/kg of feed) consisting of one of three extracts of biomass homogenate of the “remedy” beetle Alphitobius diaperinus . Extracts were: aqueous (No. 1), aqueous-methanolic (No. 2), and aqueous-methanolic after solid-phase extraction (No. 3). Extracts were immobilized on a plant-based carrier. Animals of groups 1 and 2 were kept on a standard diet without supplementation. The severe sensorimotor impairments seen in mice of group 2 (toxin) did not occur in either group 1 (controls) or groups 3–5, where animals received dietary supplementation with “antidote” consisting of darkling beetle biomass extract. The most effective was preparation No. 3.